Cloning and functional expression of the mitochondrial alternative oxidase of Aspergillus fumigatus and its induction by oxidative stress

被引：61

作者：

Magnani, T.

Soriani, F. M.

Martins, V. P.

Nascimento, A. M.

Tudella, V. G.

Curti, C.

Uyemura, S. A.

机构：

[1] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Riberiao Preto, BR-14040903 Ribeirao Preto, Brazil

[2] Univ Sao Paulo, Dept Quim Fis, Fac Ciencias Farmaceut Ribeirao Preto, BR-14049 Ribeirao Preto, Brazil

来源：

FEMS MICROBIOLOGY LETTERS | 2007年 / 271卷 / 02期

基金：

巴西圣保罗研究基金会;

关键词：

Aspergillus fumigatus; oxidative stress; alternative oxidase; heterologous expression; oxygen uptake;

D O I：

10.1111/j.1574-6968.2007.00716.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Aspergillus fumigatus possesses a branched mitochondrial electron transport chain, with both cyanide-sensitive and -insensitive oxygen-consumption activities. Mitochondrial reactive oxygen species mediate signaling for alternative oxidase (AOX) expression. A 1173 bp-long Afaox gene encoding a 40 kDa protein has been cloned and identified. Recombinant constructs containing the Afaox ORF were transformed into Escherichia coli and Saccharomyces cerevisiae for heterologous expression. In A. fumigatus, AOX activity and mRNA expression were both induced with menadione or paraquat, suggesting an important role of AOX under oxidative stress. Therefore, positive transformants showed a cyanide-resistant and salicylhydroxamic acid-sensitive respiration, whereas in control cells the oxygen uptake was completely inhibited after KCN addition.

引用

页码：230 / 238

页数：9

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