Quantitative determination of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine, 8-oxo-7,8-dihydroguanine, 8-oxo-7,8-dihydroguanosine, and their non-oxidized forms: daily concentration profile in healthy volunteers

被引：53

作者：

Andreoli, Roberta ^{[1
,2
]}

Manini, Paola ^{[1
,2
]}

De Palma, Giuseppe ^{[3
]}

Alinovi, Rossella ^{[1
]}

Goldoni, Matteo ^{[1
,2
]}

Niessen, Wilfried M. A. ^{[4
]}

Mutti, Antonio ^{[1
]}

机构：

[1] Univ Parma, Dept Clin Med Nephrol & Hlth Sci, Lab Ind Toxicol, I-43100 Parma, Italy

[2] Univ Parma, Natl Inst Occupat Safety & Prevent ISPESL, Res Ctr, I-43100 Parma, Italy

[3] Univ Brescia, Dept Expt & Appl Med Occupat Med & Ind Hyg, I-25121 Brescia, Italy

[4] Hyphen MassSpec, NL-2332 XT Leiden, Netherlands

来源：

BIOMARKERS | 2010年 / 15卷 / 03期

关键词：

Nucleic acid oxidation; 8-oxo-7,8-dihydro-2 '-deoxyguanosine; 8-oxo-7,8-dihydroguanine; 8-oxo-7,8-dihydroguanosine; LC-MS/; MS; matrix effects; creatinine; PERFORMANCE LIQUID-CHROMATOGRAPHY; OXIDATIVE DNA-DAMAGE; TANDEM MASS-SPECTROMETRY; LINKED-IMMUNOSORBENT-ASSAY; SOLID-PHASE EXTRACTION; 8-HYDROXY-2'-DEOXYGUANOSINE; REPAIR; CREATININE; DILUTION; MARKER;

D O I：

10.3109/13547500903434501

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We developed a new method for the simultaneous quantitative determination of 8-oxo-7,8-hydro-2'-deoxyguanosine (8-oxodGuo), 8-oxo-7,8-dihydroguanine (8-oxoGua), 8-oxo-7,8-dihydroguanosine (8-oxoGuo), and the corresponding non-oxidized forms, 2'-deoxyguanosine (dGuo), guanine (Gua) and guanosine (Guo), in human urine samples by liquid chromatography-tandem mass spectrometry. Differences in the ionization of analytes in different urine batches with variable matrix effects were effectively compensated for by internal standardization with stable isotope-labelled analytes. The method was sensitive enough to allow the determination of background levels of these biomarkers and was applied to characterize the inter- and intraindividual variability of biomarkers in the diurnal profile of concentrations in 24 healthy volunteers. When normalized for creatinine, none of the biomarkers was affected by sampling time, thus ruling out any circadian rhythm for nucleic acid oxidation in urine.</.

引用

页码：221 / 231

页数：11

共 48 条

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[2]

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