Determination of the molecular weight of proteins in solution from a single small-angle X-ray scattering measurement on a relative scale

被引:354
作者
Fischer, H. [2 ]
de Oliveira Neto, M. [1 ]
Napolitano, H. B. [3 ]
Polikarpov, I. [1 ]
Craievich, A. F. [2 ]
机构
[1] Univ Sao Paulo, Inst Phys Sao Carlos, BR-13560970 Sao Carlos, SP, Brazil
[2] Univ Sao Paulo, Inst Phys, BR-05314970 Sao Paulo, Brazil
[3] Univ Goias, BR-75001970 Anapolis, Go, Brazil
来源
JOURNAL OF APPLIED CRYSTALLOGRAPHY | 2010年 / 43卷
基金
巴西圣保罗研究基金会;
关键词
LOW-RESOLUTION STRUCTURES; ABSOLUTE INTENSITIES; CRYSTAL-STRUCTURES; ESCHERICHIA-COLI; COMPLEX; DIMERS;
D O I
10.1107/S0021889809043076
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
This paper describes a new and simple method to determine the molecular weight of proteins in dilute solution, with an error smaller than similar to 10%, by using the experimental data of a single small-angle X-ray scattering (SAXS) curve measured on a relative scale. This procedure does not require the measurement of SAXS intensity on an absolute scale and does not involve a comparison with another SAXS curve determined from a known standard protein. The proposed procedure can be applied to monodisperse systems of proteins in dilute solution, either in monomeric or multimeric state, and it has been successfully tested on SAXS data experimentally determined for proteins with known molecular weights. It is shown here that the molecular weights determined by this procedure deviate from the known values by less than 10% in each case and the average error for the test set of 21 proteins was 5.3%. Importantly, this method allows for an unambiguous determination of the multimeric state of proteins with known molecular weights.
引用
收藏
页码:101 / 109
页数:9
相关论文
共 28 条
[1]   The Protein Data Bank [J].
Berman, HM ;
Westbrook, J ;
Feng, Z ;
Gilliland, G ;
Bhat, TN ;
Weissig, H ;
Shindyalov, IN ;
Bourne, PE .
NUCLEIC ACIDS RESEARCH, 2000, 28 (01) :235-242
[2]   Crystal structures of human cardiac β-myosin IIS2-Δ provide insight into the functional role of the S2 subfragment [J].
Blankenfeldt, Wulf ;
Thoma, Nicolas H. ;
Wray, John S. ;
Gautel, Mathias ;
Schlichting, Ilme .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (47) :17713-17717
[3]   Domain motions and quaternary packing of phosphofructokinase-2 from Escherichia coli studied by small angle X-ray scattering and homology modeling [J].
Cabrera, R ;
Fischer, H ;
Trapani, S ;
Craievich, AF ;
Garratt, RC ;
Guixé, V ;
Babul, J .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (15) :12913-12919
[4]   Orphan nuclear receptor NGFI-B forms dimers with nonclassical interface [J].
Calgaro, Marcos R. ;
Neto, Mario de Oliveira ;
Figueira, Ana Carolina M. ;
Santos, Maria A. M. ;
Portugal, Rodrigo V. ;
Guzzi, Carolina A. ;
Saidemberg, Daniel M. ;
Bleicher, Lucas ;
Vernal, Javier ;
Fernandez, Pablo ;
Terenzi, Hernan ;
Palma, Mario Sergio ;
Polikarpov, Igor .
PROTEIN SCIENCE, 2007, 16 (08) :1762-1772
[5]   REAL-SPACE REFINEMENT OF STRUCTURE OF HEN EGG-WHITE LYSOZYME [J].
DIAMOND, R .
JOURNAL OF MOLECULAR BIOLOGY, 1974, 82 (03) :371-&
[6]   Structural analysis of N-acetylglucosamine-6-phosphate deacetylase apoenzyme from Escherichia coli [J].
Ferreira, Frederico M. ;
Mendoza-Hernandez, Guillermo ;
Castaneda-Bueno, Maria ;
Aparicio, Ricardo ;
Fischer, Hannes ;
Calcagno, Mario L. ;
Oliva, Glaucius .
JOURNAL OF MOLECULAR BIOLOGY, 2006, 359 (02) :308-321
[7]   Average protein density is a molecular-weight-dependent function [J].
Fischer, H ;
Polikarpov, I ;
Craievich, AF .
PROTEIN SCIENCE, 2004, 13 (10) :2825-2828
[8]   Low resolution structures of the retinoid X receptor DNA-binding and ligand-binding domains revealed by synchrotron x-ray solution scattering [J].
Fischer, H ;
Dias, SMG ;
Santos, MAM ;
Alves, AC ;
Zanchin, N ;
Craievich, AF ;
Apriletti, JW ;
Baxter, JD ;
Webb, P ;
Neves, FAR ;
Ribeiro, RCJ ;
Polikarpov, I .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (18) :16030-16038
[9]   COMPRESSIBILITY OF GLOBULAR-PROTEINS IN WATER AT 25-DEGREES-C [J].
GEKKO, K ;
NOGUCHI, H .
JOURNAL OF PHYSICAL CHEMISTRY, 1979, 83 (21) :2706-2714
[10]  
Glatter O., 1982, SMALL ANGLE XRAY SCA