Tgfβ2-/-Tgfβ3-/- double knockout mice display severe midline fusion defects and early embryonic lethality

被引:102
作者
Dünker, N [1 ]
Krieglstein, K [1 ]
机构
[1] Univ Gottingen, Dept Neuroanat, Ctr Anat, D-37075 Gottingen, Germany
来源
ANATOMY AND EMBRYOLOGY | 2002年 / 206卷 / 1-2期
关键词
development; rib truncation; ectopia cordis; body wall fusion; connective tissue;
D O I
10.1007/s00429-002-0273-6
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Given all known biological activities, it is anticipated that transforming growth factors beta (TGF-betas) play important roles in many different developmental processes. As all three TGF-beta isoforms display overlapping expression patterns. deletion of one TGF-beta isoform might be compensated for by another. In the present study, targeted disruption of both Tgfbeta2 and Tgfbeta3 genes was undertaken to circumvent this problem and determine the essential roles of TGF-beta2 and TGF-beta3 in vivo. Tgfbeta2(-/-)Tgfbeta3(-/-) double knockout mice and their three-allelic Tgfbeta2(-/-)Tgfbeta3(+/-) littermates display a lack of distal parts of the rib, a lack of sternal primordia, and failure in ventral body wall closure, leading to an extrathoracic position of the heart and extrusion of the liver. In addition, abnormalities in connective tissue composition and an early embryonic lethality [around embryonic day (E) 15.5] are seen. In contrast, Tgfbeta2(+/-)Tgfbeta3(-/-) littermates show normal rib and sternum development, normal anterior body wall fusion, and are still alive on E18.5. TGF-beta2 is already known to play a role in skeletal and craniofacial development. The results presented here show that beyond this: (a) TGF-betas obviously play a fundamental role in midline fusion and (b) the Tgfbeta2 gene seems to play a more important role in mediating developmental processes than the Tgfbeta3 gene, since Tgfbeta2(+/-)Tgfbeta3(-/-) mutants - in contrast to their Tgfbeta2(-/-)Tgfbeta3(+/-) littermates - do not display severe malformations.
引用
收藏
页码:73 / 83
页数:11
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