Micell-mediated extraction for the spectrophotometric determination of nitrite in water and biological samples based on its reaction with p-nitroaniline in the presence of diphenylamine

被引：79

作者：

Afkhami, A ^{[1
]}

Bahram, M ^{[1
]}

Gholami, S ^{[1
]}

Zand, Z ^{[1
]}

机构：

[1] Bu Ali Sina Univ, Fac Sci, Dept Chem, Hamadan 65174, Iran

来源：

ANALYTICAL BIOCHEMISTRY | 2005年 / 336卷 / 02期

关键词：

cloud point extraction; spectrophotometric determination; nitrite; water and biological samples;

D O I：

10.1016/j.ab.2004.10.026

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A cloud point extraction process using the nonionic surfactant Triton X-100 to extract nitrite from aqueous solution was investigated. The method is based on the color reaction of nitrite with p-nitroaniline in the presence of diphenylamine in acid media and micell-mediated extraction of an azo product. The optimal extraction and reaction conditions (e.g., acid concentration, reagent concentration. effect of time) were studied, and the analytical characteristics of the method (e.g., limit of detection, linear range, molar absorptivity, preconcentration, and improvement factors) were obtained. Linearity was obeyed in the range of 2-40 ng ml(-1) of nitrite ion. The detection limit of the method is 0.87 ng ml(-1) of nitrite ion. The interference effect of some anions and cations was also tested. The method was applied to the determination of nitrite in tap water, waste water, and human urine samples. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：295 / 299

页数：5

共 23 条

[1] Determination of traces of nitrite and nitrate in water by solid phase spectrophotometry [J].