Behavioral and neurochemical effects of the intrahippocampal co-injection of β-amyloid protein1-40 and ibotenic acid in rats

被引:12
作者
Li, Y
Qe, HQ
Chen, QS
Wang, JJ
机构
[1] Nanjing Univ, Dept Biol Sci & Technol, Nanjing 210093, Peoples R China
[2] Nanjing Med Univ, Dept Physiol, Nanjing, Peoples R China
[3] Nanjing Univ, Dept Biol Sci & Technol, Nanjing 210008, Peoples R China
基金
中国国家自然科学基金;
关键词
beta-amyloid protein1-40; ibotenic acid; learning and memory; malondialdehyde; membrane fluidity; superoxide dismutase;
D O I
10.1080/00207450490509159
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The authors investigated the effects of bilateral intrahippocampal co-injection of Abeta1-40 (4 mug for each side) with ibotenic acid (Ibo, 2 mug for each side) on rats' performance in the open field behavior, Y-maze, and passive avoidance task, and also examined some neurochemical changes in hippocampus two weeks after the co-injection. The results showed that the co-injection of Abeta1-40 with Ibo induced a decrease in exploratory activity and a significant decline in learning-memory ability of the tested rats (p<.01). The neurochemistry changes induced by the co-injection included a significant decreased in membrane fluidity of hippocampal mitochondria (p<.01), a significant decrease in the activity of SOD (p<.01), and a remarkable increase in the content of MDA (p<.01). These results suggest that the co-injection of Abeta1-40 with Ibo may induce an increase of hippocampal damage by peroxidation, and a serious learning and memory impairment of the rats. The results also suggest that the co-injection of Abeta1-40 with Ibo may provide a useful animal model for Alzheimer's disease (AD) research.
引用
收藏
页码:1521 / 1531
页数:11
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