Honokiol suppresses TNF-α-induced neutrophil adhesion on cerebral endothelial cells by disrupting polyubiquitination and degradation of IκBα

Adhesion molecules expressed on cerebral endothelial cells (ECs) mediate leukocyte recruitment and play a significant role in cerebral inflammation. Increased levels of adhesion molecules on the EC surface induce leukocyte infiltration into inflammatory areas and are thus hallmarkers of inflammation. Honokiol, isolated from the Chinese medicinal herb Magnolia officinalis, has various pharmacological activities, including anti-inflammatory effects, yet the nature of honokiol targeting molecules remains to be revealed. Here, we investigated the inhibitory effect of honokiol on neutrophil adhesion and vascular cell adhesion molecule-1 (VCAM-1) expression, which underlie its molecular target, and mechanisms for inactivating nuclear factor kappa enhancer binding protein (NF-kappa B) in mouse cerebral ECs. Honokiol inhibited tumour necrosis factor-alpha. (TNF-alpha)-induced neutrophil adhesion and VCAM-1 gene expression in cerebral ECs. The inflammatory transcription factor NF-kappa B was downregulated by honokiol. Honokiol significantly blocked TNF-alpha-induced NF-kappa B p65 nuclear translocation and degradation of the proteasome-dependent inhibitor of NF-kappa B a (1K,Bo.). From docking model prediction, honokiol directly targeted the ubiquitin-ubiquitin interface of Lys48-linked polychains. Moreover, honokiol prevented the TNF-alpha-induced Lys48-linked polyubiquitination, including I kappa B alpha-polyubiquitin interaction. Honokiol has protective anti-inflammatory effects on TNF-alpha-induced neutrophil adhesion and VCAM-1 gene expression in cerebral ECs, at least in part by directly inhibiting ubiquitination-mediated I kappa B alpha degradation and then preventing NF-kappa B nuclear translocation.