Amphiphilic polymer-liposome interaction: a novel immobilization technique for liposome on gel surface

被引:6
作者
Hara, M
Yuan, HQ
Miyake, M
Iijima, S
Yang, Q
Miyake, J
机构
[1] Minist Int Trade & Ind, Agcy Ind Sci & Technol, Natl Inst Adv Interdisciplinary Res, Tsukuba, Ibaraki 3058562, Japan
[2] Shandong Acad Med Sci, Inst Mat Med, Jinan, Shandong, Peoples R China
[3] Mitsubishi Chem Corp, Res Ctr, Midori Ku, Yokohama, Kanagawa 2260004, Japan
[4] Stanley Elect Co Ltd, Tsukuba Res Lab, Ibaraki, Osaka 3002635, Japan
来源
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2000年 / 13卷 / 1-2期
关键词
immobilization; liposome; polyacrylamide; amphiphilic polymer; gel;
D O I
10.1016/S0928-4931(00)00185-5
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
We developed a simple method for the immobilization of liposomes and biological membrane fragments to gel surface using an amphiphilic polymer, consisting of octadecylacrylamide and N,N-dimethylacrylamide with a molar ratio Of 99:1. Calcein-entrapped egg phosphatidylcholine liposomes were successfully immobilized to the surface of polyacrylamide gel containing the polymer without significant loss of entrapped calcein, Different contents of the amphiphilic polymer in the gel resulted in the different surface density of immobilized liposomes on the surface of the gel in a dose-dependent manner. As an example of biological membranes, microsome membranes from yeast cells were also well immobilized to the gel. (C) 2000 Elsevier Science S.A. All rights reserved.
引用
收藏
页码:117 / 121
页数:5
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