Dual oxidase in the intestinal epithelium of zebrafish larvae has anti-bacterial properties

被引：80

作者：

Flores, Maria Vega ^{[1
]}

Crawford, Katie C. ^{[1
]}

Pullin, Lisa M. ^{[1
]}

Hall, Christopher J. ^{[1
]}

Crosier, Kathryn E. ^{[1
]}

Crosier, Philip S. ^{[1
]}

机构：

[1] Univ Auckland, Sch Med Sci, Dept Mol Med & Pathol, Auckland 1, New Zealand

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 400卷 / 01期

关键词：

Dual oxidase; Reactive oxygen species; Intestine; Zebrafish; Inflammatory bowel disease; Innate immunity; NADPH OXIDASE; NOX/DUOX FAMILY; DIFFERENTIATION; CLONING; GROWTH;

D O I：

10.1016/j.bbrc.2010.08.037

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Reactive oxygen species (ROS) function in a range of physiological processes such as growth, metabolism and signaling, and also have a pathological role. Recent research highlighted the requirement for ROS generated by dual oxidase (DUOX) in host-defence responses in innate immunity and inflammatory disorders such as inflammatory bowel disease (IBD), but in vivo evidence to support this has, to date, been lacking. In order to investigate the involvement of Duox in gut immunity, we characterized the zebrafish ortholog of the human DUOX genes. Zebrafish duox is highly expressed in intestinal epithelial cells. Knockdown of Duox impaired larval capacity to control enteric Salmonella infection. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：164 / 168

页数：5

共 32 条

[1] Analysis of origin and growth of the thyroid gland in zebrafish [J].

Alt, Burkhard ;

Reibe, Saskia ;

Feitosa, Natalia M. ;

Elsalini, Osama A. ;

Wendl, Thomas ;

Rohr, Klaus B. .

DEVELOPMENTAL DYNAMICS, 2006, 235 (07) :1872-1883

[2] Dual oxidase in mucosal immunity and host-microbe homeostasis [J].

Bae, Yun Soo ;

Choi, Myoung Kwon ;

Lee, Won-Jae .

TRENDS IN IMMUNOLOGY, 2010, 31 (07) :278-287

[3] Cloning of two human thyroid cDNAs encoding new members of the NADPH oxidase family [J].

De Deken, X ;

Wang, DT ;

Many, MC ;

Costagliola, S ;

Libert, F ;

Vassart, G ;

Dumont, JE ;

Miot, F .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (30) :23227-23233

[4] Purification of a novel flavoprotein involved in the thyroid NADPH oxidase -: Cloning of the porcine and human cDNAs [J].

Dupuy, C ;

Ohayon, R ;

Valent, A ;

Noël-Hudson, MS ;

Dème, D ;

Virion, A .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (52) :37265-37269

[5] Dual oxidase2 is expressed all along the digestive tract [J].

El Hassani, RA ;

Benfares, N ;

Caillou, B ;

Talbot, M ;

Sabourin, JC ;

Belotte, V ;

Morand, S ;

Gnidehou, D ;

Agnandji, D ;

Ohayon, R ;

Kaniewski, J ;

Noël-Hudson, MS ;

Bidart, JM ;

Schlumberger, M ;

Virion, A ;

Dupuy, C .

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2005, 288 (05) :G933-G942

[6] Intestinal Differentiation in Zebrafish Requires Cdx1b, a Functional Equivalent of Mammalian Cdx2 [J].

Flores, Maria Vega C. ;

Hall, Chris J. ;

Davidson, Alan J. ;

Singh, Primal P. ;

Mahagaonkar, Alhad A. ;

Zon, Leonard I. ;

Crosier, Kathryn E. ;

Crosier, Philip S. .

GASTROENTEROLOGY, 2008, 135 (05) :1665-1675

[7] Dual oxidases represent novel hydrogen peroxide sources supporting mucosal surface host defense [J].

Geiszt, M ;

Witta, J ;

Baffi, J ;

Lekstrom, K ;

Leto, TL .

FASEB JOURNAL, 2003, 17 (09) :1502-+

[8] A direct role for dual oxidase in Drosophila gut immunity [J].

Ha, EM ;

Oh, CT ;

Bae, YS ;

Lee, WJ .

SCIENCE, 2005, 310 (5749) :847-850

[9] Coordination of multiple dual oxidase-regulatory pathways in responses to commensal and infectious microbes in drosophila gut [J].

Ha, Eun-Mi ;

Lee, Kyung-Ah ;

Seo, You Yeong ;

Kim, Sung-Hee ;

Lim, Jae-Hong ;

Oh, Byung-Ha ;

Kim, Jaesang ;

Lee, Won-Jae .

NATURE IMMUNOLOGY, 2009, 10 (09) :949-U19

[10] The zebrafish lysozyme C promoter drives myeloid-specific expression in transgenic fish [J].

Hall, Chris ;

Flores, Maria Vega ;

Storm, Thilo ;

Crosier, Kathy ;

Crosier, Phil .

BMC DEVELOPMENTAL BIOLOGY, 2007, 7

← 1 2 3 4 →