Bright monomeric red fluorescent protein with an extended fluorescence lifetime

被引：484

作者：

Merzlyak, Ekaterina M. ^{[1
]}

Goedhart, Joachim ^{[2
]}

Shcherbo, Dmitry ^{[3
]}

Bulina, Mariya E. ^{[3
]}

Shcheglov, Aleksandr S. ^{[3
]}

Fradkov, Arkady F. ^{[3
]}

Gaintzeva, Anna ^{[1
]}

Lukyanov, Konstantin A. ^{[3
]}

Lukyanov, Sergey ^{[3
]}

Gadella, Theodorus W. J. ^{[2
]}

Chudakov, Dmitriy M. ^{[3
]}

机构：

[1] Evrogen JSC, Moscow 117997, Russia

[2] Univ Amsterdam, Swammerdam Inst Life Sci, Sect Mol Cytol, Ctr Adv Microscopy, NL-1098 SM Amsterdam, Netherlands

[3] Shemiakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia

来源：

NATURE METHODS | 2007年 / 4卷 / 07期

关键词：

D O I：

10.1038/NMETH1062

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescent proteins have become extremely popular tools for in vivo imaging and especially for the study of localization, motility and interaction of proteins in living cells. Here we report TagRFP, a monomeric red fluorescent protein, which is characterized by high brightness, complete chromophore maturation, prolonged fluorescence lifetime and high pH-stability. These properties make TagRFP an excellent tag for protein localization studies and fluorescence resonance energy transfer (FRET) applications.

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收藏

页码：555 / 557

页数：3

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