Clinical relevance of sperm DNA damage in assisted reproduction

被引:105
作者
Tarozzi, Nicoletta
Bizzaro, Davide
Flamigni, Carlo
Andrea, Borin
机构
[1] Tecnobios Procreaz, Ctr Reprod Hlth, I-40125 Bologna, Italy
[2] Univ Polytech Marche, Inst Biol & Genet, I-60131 Ancona, Italy
[3] Univ Bologna, I-40125 Bologna, Italy
关键词
assisted reproduction outcome; diagnosis; DNAftagmentation; human spermatozoa; male infertility; treatment; protocols;
D O I
10.1016/S1472-6483(10)60678-5
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Many studies have shown how a 'paternal effect' can cause repeated assisted reproduction failures. In particular, with increasing experience of intracytoplasmic sperm injection (ICSI), it became evident that spermatozoa from some patients repeatedly fail to form viable embryos, although they can fertilize the oocyte and trigger early preimplantation development. Many authors have shown how this paternal effect can be traced back to anomalies in sperm chromatin organization: the spermatozoa of subfertile men are characterized by numerical abnormalities in spermatozoal chromosome content, Y chromosome microdeletions, alterations in the epigenetic regulation of paternal genome and non-specific DNA strand breaks. In particular, pathologically increased sperm DNA fragmentation is one of the main paternal-derived causes of repeated assisted reproduction failures in the ICSI era. The intention of this review is to describe nuclear sperm DNA damage, with emphasis on its clinical significance and its relationship with male infertility. Assessment of sperm DNA damage appears to be a potential tool for evaluating semen samples prior to their use in assisted reproduction, helping to select spermatozoa with intact DNA or with the least amount of DNA damage for use in assisted conception.
引用
收藏
页码:746 / 757
页数:12
相关论文
共 124 条
[81]  
Olsen Ann-Karin, 2005, Toxicol Appl Pharmacol, V207, P521, DOI 10.1016/j.taap.2005.01.060
[82]  
Perreault Sally D, 2003, Adv Exp Med Biol, V518, P253
[83]   ISOLATION AND CHARACTERIZATION OF A RAT CDNA CLONE ENCODING A SECRETED SUPEROXIDE-DISMUTASE REVEALS THE EPIDIDYMIS TO BE A MAJOR SITE OF ITS EXPRESSION [J].
PERRY, ACF ;
JONES, R ;
HALL, L .
BIOCHEMICAL JOURNAL, 1993, 293 :21-25
[84]   The relevance of spontaneous- and chemically-induced alterations in testicular germ cell apoptosis to toxicology [J].
Richburg, JH .
TOXICOLOGY LETTERS, 2000, 112 :79-86
[85]   An early and massive wave of germinal cell apoptosis is required for the development of functional spermatogenesis [J].
Rodriguez, I ;
Ody, C ;
Araki, K ;
Garcia, I ;
Vassalli, P .
EMBO JOURNAL, 1997, 16 (09) :2262-2270
[86]   Antioxidant treatment of patients with asthenozoospermia or moderate oligoasthenozoospermia with high-dose vitamin C and vitamin E: a randomized, placebo-controlled, double-blind study [J].
Rolf, C ;
Cooper, TG ;
Yeung, CH ;
Nieschlag, E .
HUMAN REPRODUCTION, 1999, 14 (04) :1028-1033
[87]   Bax-dependent spermatogonia apoptosis is required for testicular development and spermatogenesis [J].
Russell, LD ;
Chiarini-Garcia, H ;
Korsmeyer, SJ ;
Knudson, CM .
BIOLOGY OF REPRODUCTION, 2002, 66 (04) :950-958
[88]  
SAILER BL, 1995, J ANDROL, V16, P80
[89]   Abnormal sperm parameters in humans are indicative of an abortive apoptotic mechanism linked to the Fas-mediated pathway [J].
Sakkas, D ;
Mariethoz, E ;
St John, JC .
EXPERIMENTAL CELL RESEARCH, 1999, 251 (02) :350-355
[90]   Origin of DNA damage in ejaculated human spermatozoa [J].
Sakkas, D ;
Mariethoz, E ;
Manicardi, G ;
Bizzaro, D ;
Bianchi, PG ;
Bianchi, U .
REVIEWS OF REPRODUCTION, 1999, 4 (01) :31-37