Protein-protein interactions mapped by artificial proteases:: where σ factors bind to RNA polymerase

被引：42

作者：

Datwyler, SA ^{[1
]}

Meares, CF ^{[1
]}

机构：

[1] Univ Calif Davis, Dept Chem, Davis, CA 95616 USA

来源：

TRENDS IN BIOCHEMICAL SCIENCES | 2000年 / 25卷 / 09期

关键词：

D O I：

10.1016/S0968-0004(00)01652-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Interactions between proteins are important to understand but difficult to study. Conjugating a protein to a small artificial protease endows it with the ability to cut other proteins where it binds to them. Analysing the sites cut on the target proteins leads to new understanding of the structure of each complex. The binding of a factors to a common region on RNA polymerase provides an example.

引用

页码：408 / 414

页数：7

共 44 条

[11] CONFORMATION-DEPENDENT CLEAVAGE OF STAPHYLOCOCCAL NUCLEASE WITH A DISULFIDE-LINKED IRON CHELATE
ERMACORA, MR
DELFINO, JM
CUENOUD, B
SCHEPARTZ, A
FOX, RO
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (14) : 6383 - 6387
[12] DNAASE FOOTPRINTING - SIMPLE METHOD FOR DETECTION OF PROTEIN-DNA BINDING SPECIFICITY
GALAS, DJ
SCHMITZ, A
[J]. NUCLEIC ACIDS RESEARCH, 1978, 5 (09) : 3157 - 3170
[13] Protease activity of 1,10-phenanthroline-copper(I). Targeted scission of the catalytic site of carbonic anhydrase
Gallagher, J
Zelenko, O
Walts, AD
Sigman, DS
[J]. BIOCHEMISTRY, 1998, 37 (08) : 2096 - 2104
[14] CROSS-LINKING OF ESCHERICHIA-COLI RNA-POLYMERASE SUBUNITS - IDENTIFICATION OF BETA' AS THE BINDING-SITE OF OMEGA
GENTRY, DR
BURGESS, RR
[J]. BIOCHEMISTRY, 1993, 32 (41) : 11224 - 11227
[15] PROXIMITY MAPPING THE SURFACE OF A MEMBRANE-PROTEIN USING AN ARTIFICIAL PROTEASE - DEMONSTRATION THAT THE QUINONE-BINDING DOMAIN OF SUBUNIT-I IS NEAR THE N-TERMINAL REGION OF SUBUNIT-II OF CYTOCHROME-BD
GHAIM, JB
GREINER, DP
MEARES, CF
GENNIS, RB
[J]. BIOCHEMISTRY, 1995, 34 (36) : 11311 - 11315
[16] ESCHERICHIA-COLI-SIGMA-70 AND NUSA PROTEINS .1. BINDING INTERACTIONS WITH CORE RNA-POLYMERASE IN SOLUTION AND WITHIN THE TRANSCRIPTION COMPLEX
GILL, SC
WEITZEL, SE
VONHIPPEL, PH
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1991, 220 (02) : 307 - 324
[17] Binding of the sigma(70) protein to the core subunits of Escherichia coli RNA polymerase, studied by iron-EDTA protein footprinting
Greiner, DP
Hughes, KA
Gunasekera, AH
Meares, CF
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (01) : 71 - 75
[18] Synthesis of the protein cutting reagent iron (S)-1-(p-bromoacetamidobenzyl)ethylenediaminetetraacetate and conjugation to cysteine side chains
Greiner, DP
Miyake, R
Moran, JK
Jones, AD
Negishi, T
Ishihama, A
Meares, CF
[J]. BIOCONJUGATE CHEMISTRY, 1997, 8 (01) : 44 - 48
[19] Protein-nucleic acid interactions during open complex formation investigated by systematic alteration of the protein and DNA binding partners
Helmann, JD
deHaseth, PL
[J]. BIOCHEMISTRY, 1999, 38 (19) : 5959 - 5967
[20] HELMANN JD, 1994, TRANSCRIPTION MECH R, P1

← 1 2 3 4 5 →