Skeletal response to dietary zinc in adult female mice

被引:19
作者
Dimai, HP
Hall, SL
Stilt-Coffing, B
Farley, JR
机构
[1] Loma Linda Univ, Dept Med, Loma Linda, CA 92357 USA
[2] Loma Linda Univ, Dept Biochem, Loma Linda, CA 92357 USA
[3] Jerry L Pettis Mem Vet Adm Med Ctr, Res Serv 151, Loma Linda, CA 92357 USA
关键词
zinc; alkaline phosphatase; tartrate-resistant acid phosphatase; female mice;
D O I
10.1007/s002239900437
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The current studies were intended to assess dose- and time-dependent effects of dietary zinc (Zn) on alkaline phosphatase (ALP) activity and tartrate-resistant acid phosphatase (TRAP) activity in adult female mice. In the first study, mice were given 0, 1x, 2x, 3x, or 4x normal dietary Zn for 2 weeks, 4 weeks, or 6 weeks. In the second study, mice were given 0, Ix, 2x, 3x, 4x, and 5x normal dietary Zn for 4 weeks. Sera were collected for measurements of ALP and (in the second study) osteocalcin. Tibiae and calvaria were extracted for measurements of ALP, protein, and TRAP. The first study showed positive correlations between dietary Zn and serum ALP (4 and 6 weeks, P < 0.001), Zn and tibial ALP (2, 4, and 6 weeks, P < 0.03), and Zn and tibial protein (2, 4, and 6 weeks, P < 0.001), as well as a negative correlation between dietary Zn and tibial TRAP (2, 4, and 6 weeks, P < 0.001). Covariant analyses showed that serum ALP, tibial ALP, tibial protein, and tibial TRAP were affected by the dose of Zn (P < 0.005) and by the treatment time (P < 0.03). Supplemental studies showed that (1) the dose-dependent effect of dietary Zn on serum ALP (at 6 weeks) was proportional to the effects on tibial ALP and calvarial ALP, but not to the effects of Zn on renal, hepatic, or intestinal ALP; (2) 6 weeks of dietary Zn caused dose-dependent increases in ALP specific activity in the tibia, calvaria, and liver, but not kidneys or intestines; and (3) Zn increased ALP activity and cell layer protein and decreased TRAP activity in monolayer cultures of the murine osteoblastic cell line, MC3T3-E1. The second dietary study confirmed the results of the first: 4 weeks of treatment with Zn caused significant increases in serum ALP, calvarial ALP, and tibial ALP activities, and a significant decrease in tibial TRAP (P < 0.05-0.005 for each). This study also revealed an effect of Zn to increase serum osteocalcin (P < 0.03 at 2x normal Zn). Together, these data indicate that incremental increases in dietary Zn are associated with increases in ALP activity in serum and in bone. The effect of Zn to decrease TRAP activity in osteoblast-line cells precludes the interpretation of a Zn-dependent decrease in tibial TRAP activity as evidence of decreased bone resorption.
引用
收藏
页码:309 / 315
页数:7
相关论文
共 39 条
  • [31] Nishi Y, 1996, J AM COLL NUTR, V15, P340
  • [32] INVITRO DIFFERENTIATION AND CALCIFICATION IN A NEW CLONAL OSTEOGENIC CELL-LINE DERIVED FROM NEWBORN MOUSE CALVARIA
    SUDO, H
    KODAMA, HA
    AMAGAI, Y
    YAMAMOTO, S
    KASAI, S
    [J]. JOURNAL OF CELL BIOLOGY, 1983, 96 (01) : 191 - 198
  • [33] VALEE BL, 1983, ZINC ENZYMES, P1
  • [34] BONE-ALKALINE PHOSPHATASE AS INDICATOR OF BONE-FORMATION
    VANSTRAALEN, JP
    SANDERS, E
    PRUMMEL, MF
    SANDERS, GTB
    [J]. CLINICA CHIMICA ACTA, 1991, 201 (1-2) : 27 - 34
  • [35] WALLWORK JC, 1990, NUTRITION BONE DEV, P316
  • [36] A MISSENSE MUTATION IN THE HUMAN-LIVER BONE KIDNEY ALKALINE-PHOSPHATASE GENE CAUSING A LETHAL FORM OF HYPOPHOSPHATASIA
    WEISS, MJ
    COLE, DEC
    RAY, K
    WHYTE, MP
    LAFFERTY, MA
    MULIVOR, RA
    HARRIS, H
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (20) : 7666 - 7669
  • [37] ACTION OF ZINC ON BONE METABOLISM IN RATS - INCREASES IN ALKALINE-PHOSPHATASE ACTIVITY AND DNA CONTENT
    YAMAGUCHI, M
    YAMAGUCHI, R
    [J]. BIOCHEMICAL PHARMACOLOGY, 1986, 35 (05) : 773 - 777
  • [38] STIMULATORY EFFECT OF ZINC ON BONE-FORMATION IN TISSUE-CULTURE
    YAMAGUCHI, M
    OISHI, H
    SUKETA, Y
    [J]. BIOCHEMICAL PHARMACOLOGY, 1987, 36 (22) : 4007 - 4012
  • [39] YAMAGUCHI M, JPN J BONE MINER RES, V2, P186