A new optical sensing reaction for nitric oxide

被引:22
作者
Dacres, H [1 ]
Narayanaswamy, R [1 ]
机构
[1] Univ Manchester, Dept Instrumentat & Analyt Sci, Manchester M60 1QD, Lancs, England
来源
SENSORS AND ACTUATORS B-CHEMICAL | 2003年 / 90卷 / 1-3期
关键词
nitric oxide; absorbance; quantitation; silicone rubber; eriochrome cyanine R;
D O I
10.1016/S0925-4005(03)00032-7
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Based on the reaction between the Cu(II) complex of Eriochrome cyanine R (ECR) and nitric oxide (NO) in phosphate buffer (pH 7.4), a new colorimetric method for the determination of NO concentration has been developed. The linear calibration range for NO was 0-60 muM with a detection limit of 1.24 muM. This reaction was then used as the basis in the development of a fibre optic chemical sensor for NO gas. The copper complex was incorporated into silicone rubber membranes and exposed to NO gas after incubating the films in phosphate buffer (pH 7.4). A linear calibration for NO gas between 0 and 6 ppm was obtained with a detection limit of 0.227 ppm (1 muM similar or equal to 0.031 ppm NO in solution). The sensor response was shown to be reproducible and reversible (2.77%, R.S.D., n = 4) upon exposure to aqueous phosphate buffer (pH 7.4). The sensor response was also found to be pH independent between 7 and 10. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:222 / 229
页数:8
相关论文
共 32 条
[1]   Electron transfer, oxygen binding, and nitric oxide feedback inhibition in endothelial nitric-oxide synthase [J].
Abu-Soud, HM ;
Ichimori, K ;
Presta, A ;
Stuehr, DJ .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (23) :17349-17357
[2]   Hydrophobic membrane sensors for the optical determination of hydrogen chloride gas [J].
Baron, MG ;
Narayanaswamy, R ;
Thorpe, SC .
SENSORS AND ACTUATORS B-CHEMICAL, 1996, 34 (1-3) :511-515
[3]  
Christian GD., 1986, Instrumental Analysis
[4]   DIRECT MEASUREMENT OF THE ACCUMULATION AND MITOCHONDRIAL CONVERSION OF NITRIC-OXIDE WITHIN CHINESE-HAMSTER OVARY CELLS USING AN INTRACELLULAR ELECTRON-PARAMAGNETIC-RESONANCE TECHNIQUE [J].
CLARKSON, RB ;
NORBY, SW ;
SMIRNOV, A ;
BOYER, S ;
VAHIDI, N ;
NIMS, RW ;
WINK, DA .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1995, 1243 (03) :496-502
[5]   Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: Implications for intracellular measurement of reactive nitrogen and oxygen species [J].
Crow, JP .
NITRIC OXIDE-BIOLOGY AND CHEMISTRY, 1997, 1 (02) :145-157
[6]   THE OBLIGATORY ROLE OF ENDOTHELIAL-CELLS IN THE RELAXATION OF ARTERIAL SMOOTH-MUSCLE BY ACETYLCHOLINE [J].
FURCHGOTT, RF ;
ZAWADZKI, JV .
NATURE, 1980, 288 (5789) :373-376
[7]   THE REACTION OF NITRIC-OXIDE WITH COPPER PROTEINS AND THE PHOTODISSOCIATION OF COPPER-NO COMPLEXES [J].
GORREN, ACF ;
DEBOER, E ;
WEVER, R .
BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 916 (01) :38-47
[8]  
Grisham Matthew B., 1995, Methods (Orlando), V7, P84, DOI 10.1006/meth.1995.1012
[9]  
Keefer LK, 1996, METHOD ENZYMOL, V268, P281
[10]   A SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF NITRIC-OXIDE [J].
KINOSHITA, S ;
WAKITA, H ;
MASUDA, I .
ANALYTICA CHIMICA ACTA, 1985, 169 (MAR) :373-376