A new optical sensing reaction for nitric oxide

被引：22

作者：

Dacres, H ^{[1
]}

Narayanaswamy, R ^{[1
]}

机构：

[1] Univ Manchester, Dept Instrumentat & Analyt Sci, Manchester M60 1QD, Lancs, England

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2003年 / 90卷 / 1-3期

关键词：

nitric oxide; absorbance; quantitation; silicone rubber; eriochrome cyanine R;

D O I：

10.1016/S0925-4005(03)00032-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Based on the reaction between the Cu(II) complex of Eriochrome cyanine R (ECR) and nitric oxide (NO) in phosphate buffer (pH 7.4), a new colorimetric method for the determination of NO concentration has been developed. The linear calibration range for NO was 0-60 muM with a detection limit of 1.24 muM. This reaction was then used as the basis in the development of a fibre optic chemical sensor for NO gas. The copper complex was incorporated into silicone rubber membranes and exposed to NO gas after incubating the films in phosphate buffer (pH 7.4). A linear calibration for NO gas between 0 and 6 ppm was obtained with a detection limit of 0.227 ppm (1 muM similar or equal to 0.031 ppm NO in solution). The sensor response was shown to be reproducible and reversible (2.77%, R.S.D., n = 4) upon exposure to aqueous phosphate buffer (pH 7.4). The sensor response was also found to be pH independent between 7 and 10. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：222 / 229

页数：8