A new plasmid-encoded proteic killer gene system: Cloning, sequencing, and analysing hig locus of plasmid Rts1

被引：69

作者：

Tian, QB ^{[1
]}

Ohnishi, M ^{[1
]}

Tabuchi, A ^{[1
]}

Terawaki, Y ^{[1
]}

机构：

[1] SHINSHU UNIV,SCH MED,DEPT BACTERIOL,MATSUMOTO,NAGANO 390,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 220卷 / 02期

关键词：

D O I：

10.1006/bbrc.1996.0396

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new proteic killer gene system, hig, was identified on the plasmid Rts1. The hig locus consisting of higA and higB is directly related to the temperature sensitive host cell growth conferred by Rts1. We proved that higB encoding presumably a 92-amino-acid polypeptide inhibited segregation of plasmid free cells, and higA encoding a 104-amino-acid polypeptide suppressed the HigB function both in cis and in trans. (C) 1996 Academic Press, Inc.

引用

页码：280 / 284

页数：5

共 22 条

[11] CONTROL OF CELL-DIVISION BY SEX FACTOR-F IN ESCHERICHIA-COLI .1. THE 42.84-43.6 F SEGMENT COUPLES CELL-DIVISION OF THE HOST BACTERIA WITH REPLICATION OF PLASMID DNA [J].

MIKI, T ;

YOSHIOKA, K ;

HORIUCHI, T .

JOURNAL OF MOLECULAR BIOLOGY, 1984, 174 (04) :605-625

[12] 3 SHORT FRAGMENTS OF RTS1 DNA ARE RESPONSIBLE FOR THE TEMPERATURE-SENSITIVE GROWTH PHENOTYPE (TSG) OF HOST BACTERIA [J].

MOCHIDA, S ;

TSUCHIYA, H ;

MORI, K ;

KAJI, A .

JOURNAL OF BACTERIOLOGY, 1991, 173 (08) :2600-2607

[13] MINI-F PLASMID GENES THAT COUPLE HOST-CELL DIVISION TO PLASMID PROLIFERATION [J].

OGURA, T ;

HIRAGA, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (15) :4784-4788

[14] IDENTIFICATION OF THE RTS-1 DNA FRAGMENT RESPONSIBLE FOR TEMPERATURE SENSITIVE GROWTH OF HOST-CELLS HARBORING A DRUG-RESISTANCE FACTOR-RTS-1 [J].

OKAWA, N ;

TANAKA, M ;

FINVER, S ;

KAJI, A .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 142 (03) :1084-1088

[15] THE PARDE OPERON OF THE BROAD-HOST-RANGE PLASMID RK2 SPECIFIES GROWTH-INHIBITION ASSOCIATED WITH PLASMID LOSS [J].

ROBERTS, RC ;

STROM, AR ;

HELINSKI, DR .

JOURNAL OF MOLECULAR BIOLOGY, 1994, 237 (01) :35-51

[16] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].

SANGER, F ;

NICKLEN, S ;

COULSON, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467

[17] REPLICATION OF R-FACTOR RTS1 IN PROTEUS-MIRABILIS [J].

TERAWAKI, Y ;

ROWND, R .

JOURNAL OF BACTERIOLOGY, 1972, 109 (02) :492-&

[18] TEMPERATURE SENSITIVITY OF CELL GROWTH IN ESCHERICHIA COLI ASSOCIATED WITH TEMPERATURE SENSITIVE R(KM) FACTOR [J].

TERAWAKI, Y ;

KAKIZAWA, Y ;

TAKAYASU, H ;

YOSHIKAWA, M .

NATURE, 1968, 219 (5151) :284-+

[19] AN RTS1-DERIVATIVE PLASMID CONFERRING UV SENSITIVITY ON ESCHERICHIA-COLI HOST [J].

TERAWAKI, Y ;

KOBAYASHI, Y ;

MATSUMOTO, H ;

KAMIO, Y .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1980, 97 (02) :694-699

[20] 2 GENES, PEMK AND PEMI, RESPONSIBLE FOR STABLE MAINTENANCE OF RESISTANCE PLASMID R100 [J].

TSUCHIMOTO, S ;

OHTSUBO, H ;

OHTSUBO, E .

JOURNAL OF BACTERIOLOGY, 1988, 170 (04) :1461-1466

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