A nucleotide binding motif in hepatitis C virus (HCV) NS4B mediates HCV RNA replication

被引：90

作者：

Einav, S

Elazar, M

Danieli, T

Glenn, JS

机构：

[1] Stanford Univ, Sch Med, Div Gastroenterol & Hepatol, Stanford, CA 94305 USA

[2] Vet Adm Med Ctr, Palo Alto, CA 94304 USA

[3] Hebrew Univ Jerusalem, Alexander Silberman Inst Life Sci, Wolfson Ctr Appl Struct Biol, Jerusalem, Israel

来源：

JOURNAL OF VIROLOGY | 2004年 / 78卷 / 20期

关键词：

D O I：

10.1128/JVI.78.20.11288-11295.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Hepatitis C virus (HCV) is a major cause of viral hepatitis. There is no effective therapy for most patients. We have identified a nucleotide binding motif (NBM) in one of the virus's nonstructural proteins, NS4B. This structural motif binds and hydrolyzes GTP and is conserved across HCV isolates. Genetically disrupting the NBM impairs GTP binding and hydrolysis and dramatically inhibits HCV RNA replication. These results have exciting implications for the HCV life cycle and novel antiviral strategies.

引用

页码：11288 / 11295

页数：8

共 33 条

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