A new imprinted cluster on the human chromosome 7q21-q31, identified by human-mouse monochromosomal hybrids

被引：68

作者：

Okita, C ^{[1
]}

Meguro, M ^{[1
]}

Hoshiya, H ^{[1
]}

Haruta, M ^{[1
]}

Sakamoto, YK ^{[1
]}

Oshimura, M ^{[1
]}

机构：

[1] Tottori Univ, Fac Med, Sch Life Sci, Dept Mol & Cellular Biol, Tottori 6838503, Japan

来源：

GENOMICS | 2003年 / 81卷 / 06期

关键词：

D O I：

10.1016/S0888-7543(03)00052-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have previously established a series of human monochromosomal hybrids containing a single human chromosome of defined parental origin as an in vitro resource for the investigation of human imprinted loci. Using the hybrids with a paternal or maternal human chromosome 7, we determined the allelic expression profiles of 76 ESTs mapped to the human chromosome 7q21-q31. Seven genes/transcripts, including PEG10 which has previously been reported to be imprinted, showed parent-of-origin-specific expression in monochromosomal hybrids. One of the 6 candidate genes/transcripts, i.e., DLX5 was confirmed to be imprinted in normal human lymphoblasts and brain tissues by a polymorphic analysis. Thus, an imprinted domain has been newly defined in the region of human chromosome 7q21-q31 using human-mouse monochromosomal hybrids. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：556 / 559

页数：4

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