Cinnamic acid inhibits Zika virus by inhibiting RdRp activity

被引：45

作者：

Chen, Yuting ^{[1
]}

Li, Zhaoxin ^{[2
]}

Pan, Pan ^{[3
]}

Lao, Zizhao ^{[4
]}

Xu, Jiangtao ^{[2
]}

Li, Zonghui ^{[2
]}

Zhan, Shaofeng ^{[5
]}

Liu, Xiaohong ^{[5
]}

Wu, Yina ^{[1
]}

Wang, Wenbiao ^{[6
]}

Li, Geng ^{[2
]}

机构：

[1] Guangzhou Univ Chinese Med, Sch Pharmaceut Sci, Guangzhou 510006, Peoples R China

[2] Guangzhou Univ Chinese Med, Lab Anim Ctr, Guangzhou 510006, Peoples R China

[3] Jinan Univ, Affiliated Hosp 1, Guangzhou 510632, Peoples R China

[4] Guangzhou Univ Chinese Med, Math Engn Acad Chinese Med, Guangzhou 510006, Peoples R China

[5] Guangzhou Univ Chinese Med, Affiliated Hosp 1, Guangzhou 510000, Peoples R China

[6] Jinan Univ, Inst Med Microbiol, Guangdong Prov Key Lab Virol, Guangzhou 510632, Peoples R China

来源：

ANTIVIRAL RESEARCH | 2021年 / 192卷

基金：

中国国家自然科学基金;

关键词：

Cinnamic acid; ZIKV; RdRp; In vitro; In vivo; ANTIVIRAL ACTIVITY; INFECTION; REPLICATION; CELLS; IMMUNOGENICITY; PHASE-1; SCREEN; SAFETY;

D O I：

10.1016/j.antiviral.2021.105117

中图分类号：

R9 [药学];

学科分类号：

100702 [药剂学];

摘要：

In recent years, Zika virus (ZIKV), which causes severe diseases such as congenital microcephaly and Guillain-Barr 'e syndrome, bringing serious harm to humans, has spread throughout the world. However, there are currently no effective drugs against the virus, and the need to develop anti-ZIKV drugs is thus urgent. In this study, we evaluated the antiviral efficacy of cinnamic acid against ZIKV by using reverse transcription-quantitative real-time PCR (qRT-PCR), plaque-forming, immunofluorescence and Western blotting. Additionally, Cinnamic acid possessed anti-ZIKV properties against the post-entry stage of the ZIKV replication cycle, and inhibited RdRp activity. In vivo, we found that cinnamic acid reduced the mortality of mice, viral load in the blood and ZIKV protein levels in the brain. Based on our experiments, cinnamic acid was found to be a potential effective anti-ZIKV drug.

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页数：8

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