Antibody engineering

被引：174

作者：

Maynard, J ^{[1
]}

Georgiou, G

机构：

[1] Univ Texas, Dept Chem Engn, Austin, TX 78712 USA

[2] Univ Texas, Inst Mol & Cellular Biol, Austin, TX 78712 USA

来源：

ANNUAL REVIEW OF BIOMEDICAL ENGINEERING | 2000年 / 2卷

关键词：

phage display; library screening; scFv; antigen; combinatorial libraries;

D O I：

10.1146/annurev.bioeng.2.1.339

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Antibodies are unique in their high affinity and specificity for a binding partner, a quality that has made them one of the most useful molecules for biotechnology and biomedical applications. The field of antibody engineering has changed rapidly in the past 10 years, fueled by novel technologies for the in vitro isolation of antibodies from combinatorial libraries and their functional expression in bacteria. This review presents an overview of the methods available for the de novo generation of human antibodies, for engineering antibodies with increased antigen affinity, and for the production of antibody fragments. Select applications of recombinant antibodies are also presented.

引用

页码：339 / 376

页数：38

共 234 条

[1] ENGINEERING ANTIBODIES FOR THERAPY [J].