Sensory rhodopsin II transducer HtrII is also responsible for serine chemotaxis in the archaeon Halobacterium salinarum

被引：33

作者：

Hou, SB ^{[1
]}

Brooun, A ^{[1
]}

Yu, HS ^{[1
]}

Freitas, T ^{[1
]}

Alam, M ^{[1
]}

机构：

[1] Univ Hawaii, Dept Microbiol, Honolulu, HI 96822 USA

来源：

JOURNAL OF BACTERIOLOGY | 1998年 / 180卷 / 06期

关键词：

D O I：

10.1128/JB.180.6.1600-1602.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Previously, we demonstrated that the methyl-accepting protein HtrII is the transducer for photoreceptor sensory rhodopsin H. Here, we provide experimental evidence that HtrII is also a chemotransducer. Using an agarose-in-plug bridge method, we show that an HtrII overexpression strain has a quicker response to serine than does an HtrII deletion strain. Furthermore, an in vivo flow assay demonstrates that the deletion strain is unable to modulate methylesterase activity after serine addition or photostimulation, while the overexpression strain shows distinct methanol peaks following both types of stimuli.

引用

页码：1600 / 1602

页数：3

共 13 条

[11] The primary structures of the Archaeon Halobacterium salinarium blue light receptor sensory rhodopsin II and its transducer, a methyl-accepting protein [J].