Modified screen-printed electrodes for the investigation of the interaction of non-electroactive quinazoline derivatives with DNA

被引：36

作者：

Labuda, J ^{[1
]}

Bucková, M

Jantová, S

Stepánek, I

Surugiu, I

Danielsson, B

Mascini, M

机构：

[1] Slovak Univ Technol Bratislava, Dept Analyt Chem, SK-81237 Bratislava, Slovakia

[2] Slovak Univ Technol Bratislava, Dept Microbiol Biochem & Biol, SK-81237 Bratislava, Slovakia

[3] FACH, SK-08006 Presov, Slovakia

[4] Lund Univ, Dept Pure & Appl Biochem, S-22100 Lund, Sweden

[5] Univ Florence, Dept Publ Hlth Environm & Analyt Chem, I-50121 Florence, Italy

来源：

FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 2000年 / 367卷 / 04期

关键词：

D O I：

10.1007/s002160000388

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Five morpholino-quinazoline derivatives have bern investigated voltammetrically using a competition with the tris(o-phenanthroline) cobalt(III) redox marker for the accumulation at dsDNA modified screen-printed electrodes. An association of quinazolines with DNA was observed at the modified electrodes polarized by the negative potential of -0.4 V vs. AE/AgCl. This was confirmed by a potentiometric stripping analysis based on the DNA guanine signal. Calibration curves for quinazolines within a concentration range of mu mol/L were obtained with DP voltammetry using 5 x 10(-7) mol/L Co(phen)(3)(3+) marker. The quinazolines exhibit no effect on the DNA complex with the fluorescent thiazole orange derivative TO-PRO-3. The role of the accumulation potential in the association interaction with DNA is discussed.

引用

页码：364 / 368

页数：5

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