Cryptosporidium parvum infection in bovine neonates: dynamic clinical, parasitic and immunologic patterns

被引:128
作者
Fayer, R [1 ]
Gasbarre, L
Pasquali, P
Canals, A
Almeria, S
Zarlenga, D
机构
[1] USDA, ARS, Immunol & Dis Resistance Lab, Beltsville, MD 20705 USA
[2] Univ Perugia, Fac Med Vet, Ist Parassitol, I-06100 Perugia, Italy
关键词
Cryptosporidium parvum; cryptosporidiosis; bovine; cattle; prevalence; diarrhoea; immunity; cytokine;
D O I
10.1016/S0020-7519(97)00170-7
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Twenty-six experimentally infected calves were monitored daily for oocyst excretion. All began excreting oocysts 3-6 days p.i. Most calves (n = 23) excreted oocysts for 6-9 days, with a daily range from 4 x 10(2) to 4.15 x 10(7) oocysts g(-1) of faeces. Over half the calves excreted peak numbers of oocysts 6-8 days p.i. Diarrhoea, observed intermittently beginning as early as day 3 p.i., lasted 4-16 days and varied greatly in severity from calf to calf. In a second study, nine of 18 calves were orally inoculated with 5 x 10(6) oocysts between birth and 2 days of age and nine remained uninfected. Monoclonal antibodies for cell surface markers indicated substantial increases in CD4+ and CD8+ T cells in the intraepithelial lymphocyte population of the ilea of infected calves at 7-9 days of age. RT-PCR demonstrated increases in mRNA for interleukin-12 and interferon-gamma that correlated with increases in both CD4+ and CD8+ intraepithelial lymphocyte cells. Increased mRNA for interleukin-12 and interferon-gamma from lamina propria lymphocytes correlated with increased numbers of CD8 + cells. No changes were found in interleukin-2, interleukin-4 or interleukin-10 mRNA levels. However, interleukin-15 mRNA, possibly from epithelial cells contaminating intraepithelial lymphocytes, was decreased in infected calves and had a negative correlation with increases in CD4+ and CD8+ cells. No differences were detected in mRNA levels for cytokines from lymph node lymphocytes. Published by Elsevier Science Ltd on behalf of the Australian Society for Parasitology.
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收藏
页码:49 / 56
页数:8
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