Reproducibility of AMPLICOR enterovirus PCR test results

被引：20

作者：

Rotbart, HA

机构：

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1997年 / 35卷 / 12期

关键词：

D O I：

10.1128/JCM.35.12.3301-3302.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The reproducibility of AMPLICOR enterovirus PCR test results was determined with clinical samples of cerebrospinal fluid, serum, urine, and throat and rectal swabs. Among 608 samples from which duplicate aliquots were run simultaneously, only seven pairs gave discordant results, Among 104 samples from which duplicate aliquots were run in separate assays, no discordance was seen. Overall, the reproducibility of test kit results was 99% (705 of 712).

引用

收藏

页码：3301 / 3302

页数：2

相关论文

共 9 条

[1] CLINICAL AND LABORATORY OBSERVATIONS - DIAGNOSIS OF NEONATAL ENTEROVIRUS INFECTION BY POLYMERASE CHAIN-REACTION [J].

ABZUG, MJ ;

LOEFFELHOLZ, M ;

ROTBART, HA .

JOURNAL OF PEDIATRICS, 1995, 126 (03) :447-450

[2] Nonpolio enteroviruses and the febrile young infant: Epidemiologic, clinical and diagnostic aspects [J].

Dagan, R .

PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1996, 15 (01) :67-71

[3] AVOIDING FALSE POSITIVES WITH PCR [J].

KWOK, S ;

HIGUCHI, R .

NATURE, 1989, 339 (6221) :237-238

[4] Detection of enteroviruses by polymerase chain reaction in urine samples of patients with aseptic meningitis [J].

Nielsen, LP ;

Modlin, JF ;

Rotbart, HA .

PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1996, 15 (07) :625-627

[5]

Romero Jose R., 1996, Pediatric Research, V39, p139A

[6] Diagnosis of enterovirus infection by polymerase chain reaction of multiple specimen types [J].

Rotbart, HA ;

Ahmed, A ;

Hickey, S ;

Dagan, R ;

McCracken, GH ;

Whitley, RJ ;

Modlin, JF ;

Cascino, M ;

OConnell, JF ;

Menegus, MA ;

Blum, D .

PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1997, 16 (04) :409-411

[7] DIAGNOSIS OF ENTEROVIRAL MENINGITIS BY USING PCR WITH A COLORIMETRIC MICROWELL DETECTION ASSAY [J].

ROTBART, HA ;

SAWYER, MH ;

FAST, S ;

LEWINSKI, C ;

MURPHY, N ;

KEYSER, EF ;

SPADORO, J ;

KAO, SY ;

LOEFFELHOLZ, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (10) :2590-2592

[8]

Rotbart Harley A., 1995, P271

[9]

SCHLESINGER Y, 1994, PEDIATRICS, V94, P157