Lipid transmembrane asymmetry and intrinsic membrane potential: Two sides of the same coin

被引:77
作者
Gurtovenko, Andrey A. [1 ]
Vattulainen, Ilpo
机构
[1] Univ Bradford, Inst Pharmaceut Innovat, Computat Lab, Bradford BD7 1DP, W Yorkshire, England
[2] Tampere Univ Technol, Inst Phys, FI-33101 Tampere, Finland
[3] Aalto Univ, FI-02015 Helsinki, Finland
[4] Univ So Denmark, Ctr Biomembrane Phys, MEMPHYS, Odense, Denmark
关键词
D O I
10.1021/ja070949m
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
To better understand the origin of intrinsic membrane potential in cells, we have performed atomic-scale molecular dynamics simulations of an asymmetric lipid membrane comprised of zwitterionic (neutral) phosphatidylcholine (PC) and phosphatidylethanolamine (PE) leaflets in the absence of salt. It turns out that the asymmetry in distribution of zwitterionic lipids across the membrane gives rise to a nonzero intrinsic potential difference of about 100 mV between the two sides of the membrane. This potential arises from the difference in dipole moments of the two leaflets of the asymmetric PC/PE membrane. Our findings suggest that the transmembrane lipid asymmetry typical of most living cells can contribute to the membrane potential.
引用
收藏
页码:5358 / +
页数:3
相关论文
共 16 条
[1]   Aminophospholipid asymmetry: A matter of life and death [J].
Balasubramanian, K ;
Schroit, AJ .
ANNUAL REVIEW OF PHYSIOLOGY, 2003, 65 :701-734
[2]   Molecular dynamics simulations of a fluid bilayer of dipalmitoylphosphatidylcholine at full hydration, constant pressure, and constant temperature [J].
Berger, O ;
Edholm, O ;
Jahnig, F .
BIOPHYSICAL JOURNAL, 1997, 72 (05) :2002-2013
[3]   The implementation of slab geometry for membrane-channel molecular dynamics simulations [J].
Bostick, D ;
Berkowitz, ML .
BIOPHYSICAL JOURNAL, 2003, 85 (01) :97-107
[4]  
BURTOVENKO AA, 2005, J CHEM PHYS, V122
[5]  
BURTOVENKO AA, 2005, J CHEM PHYS, V122
[6]  
Gennis R.B., 1989, BIOMEMBRANES MOL STR
[7]   Pore formation coupled to ion transport through lipid membranes as induced by transmembrane ionic charge imbalance: Atomistic molecular dynamics study [J].
Gurtovenko, AA ;
Vattulainen, I .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (50) :17570-17571
[8]   Ion leakage through transient water pores in protein-free lipid membranes driven by transmembrane ionic charge imbalance [J].
Gurtovenko, Andrey A. ;
Vattulainen, Ilpo .
BIOPHYSICAL JOURNAL, 2007, 92 (06) :1878-1890
[9]   DIPOLE POTENTIAL MEASUREMENTS IN ASYMMETRIC MEMBRANES [J].
LATORRE, R ;
HALL, JE .
NATURE, 1976, 264 (5584) :361-363
[10]   GROMACS 3.0: a package for molecular simulation and trajectory analysis [J].
Lindahl, E ;
Hess, B ;
van der Spoel, D .
JOURNAL OF MOLECULAR MODELING, 2001, 7 (08) :306-317