Molecular detection of Histoplasma capsulatum var. capsulatum in human clinical samples

被引：59

作者：

Bracca, A

Tosello, ME

Girardini, JE

Amigot, SL

Gomez, C

Serra, E

机构：

[1] Consejo Nacl Invest Cient & Tecn, Inst Biol Mol & Celular Rosario, IBR, Fac Ciencias Bioquim & Farmaceut, RA-2000 Rosario, Santa Fe, Argentina

[2] CEREMIC, Ctr Referencia Micol, RA-2000 Rosario, Santa Fe, Argentina

[3] Municipalidad Rosario, Direcc Labs, RA-2000 Rosario, Santa Fe, Argentina

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 04期

关键词：

D O I：

10.1128/JCM.41.4.1753-1755.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed a seminested PCR for the diagnosis of histoplasmosis that amplifies a portion of the Histoplasma capsulatum H antigen gene. This assay is highly sensitive and specific, being able to detect genomic material corresponding to less than 10 yeast cells without cross-reaction against other bacterial or fungal pathogens.

引用

页码：1753 / 1755

页数：3