Cortical capture of microtubules and spindle polarity in budding yeast - where's the catch?

被引:44
作者
Huisman, SM [1 ]
Segal, M [1 ]
机构
[1] Univ Cambridge, Dept Genet, Cambridge CB2 3EH, England
关键词
microtubules; spatial cues; cell polarity; cell cycle; budding yeast;
D O I
10.1242/jcs.01650
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In asymmetric divisions, the mitotic spindle must align according to the cell polarity axis. This is achieved through targeting astral microtubules emanating from each spindle pole to opposite cell cortex compartments. Saccharomyces cerevisiae is a powerful genetic model for dissection of this complex process. Intense research in this yeast has rendered detailed models for a program linking actin organization and spindle orientation along the mother-bud axis. This program requires the separate contributions of Kar9p, a protein guiding microtubules along polarized actin cables, and the polarity determinant Bud6p/Aip3 that marks sites for cortical capture at the bud tip and bud neck. In an added layer of complexity, cyclin-dependent kinase (Cdk) differentially regulates spindle pole function to dictate asymmetric spindle pole fate. Asymmetric contacts established by the spindle poles impart a further layer of extrinsic asymmetry restricting recruitment of Kar9p to the pole destined for the daughter cell. As a result, astral microtubules from a single pole are guided to the bud compartment after spindle assembly. Finally, Cdk might also translocate along astral microtubules in association with Kar9p to modulate microtubule-cortex interactions following spindle alignment. Insertion of the mitotic spindle into the bud neck is driven by the microtubule motor dynein. This process relies on the combined action of microtubule-plus-end-tracking proteins and kinesins that control the cell-cycle-dependent abundance of dynein at microtubule plus ends. Thus, this actin-independent pathway for spindle orientation might also be influenced by Cdk.
引用
收藏
页码:463 / 471
页数:9
相关论文
共 92 条
[21]   Formins: signaling effectors for assembly and polarization of actin filaments [J].
Evangelista, M ;
Zigmond, S ;
Boone, C .
JOURNAL OF CELL SCIENCE, 2003, 116 (13) :2603-2611
[22]   Bni1p, a yeast formin linking Cdc42p and the actin cytoskeleton during polarized morphogenesis [J].
Evangelista, M ;
Blundell, K ;
Longtine, MS ;
Chow, CJ ;
Adames, N ;
Pringle, JR ;
Peter, M ;
Boone, C .
SCIENCE, 1997, 276 (5309) :118-122
[23]   Cortical Num1p interacts with the dynein intermediate chain Pac11p and cytoplasmic microtubules in budding yeast [J].
Farkasovsky, M ;
Küntzel, H .
JOURNAL OF CELL BIOLOGY, 2001, 152 (02) :251-262
[24]   Regulation of a formin complex by the microtubule plus end protein tea1p [J].
Feierbach, B ;
Verde, F ;
Chang, F .
JOURNAL OF CELL BIOLOGY, 2004, 165 (05) :697-707
[25]   Role of bud6p and tea1p in the interaction between actin and microtubules for the establishment of cell polarity in fission yeast [J].
Glynn, JM ;
Lustig, RJ ;
Berlin, A ;
Chang, F .
CURRENT BIOLOGY, 2001, 11 (11) :836-845
[26]   Synthetic lethal analysis implicates Ste20p, a p21-activated protein kinase, in polarisome activation [J].
Goehring, AS ;
Mitchell, DA ;
Tong, AHY ;
Keniry, ME ;
Boone, C ;
Sprague, GF .
MOLECULAR BIOLOGY OF THE CELL, 2003, 14 (04) :1501-1516
[27]   Cortical control of microtubule stability and polarization [J].
Gundersen, GG ;
Gomes, ER ;
Wen, Y .
CURRENT OPINION IN CELL BIOLOGY, 2004, 16 (01) :106-112
[28]   β-tubulin C354 mutations that severely decrease microtubule dynamics do not prevent nuclear migration in yeast [J].
Gupta, ML ;
Bode, CJ ;
Thrower, DA ;
Pearson, CG ;
Suprenant, KA ;
Bloom, KS ;
Himes, RH .
MOLECULAR BIOLOGY OF THE CELL, 2002, 13 (08) :2919-2932
[29]   A journey into space [J].
Hayles, J ;
Nurse, P .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2001, 2 (09) :647-656
[30]   The cortical protein Num1p is essential for dynein-dependent interactions of microtubules with the cortex [J].
Heil-Chapdelaine, RA ;
Oberle, JR ;
Cooper, JA .
JOURNAL OF CELL BIOLOGY, 2000, 151 (06) :1337-1343