Transplantation of cultured bone cells using combinations of scaffolds and culture techniques

被引：117

作者：

Uemura, T

Dong, J

Wang, YC

Kojima, H

Saito, T

Iejima, D

Kikuchi, M

Tanaka, J

Tateishi, T

机构：

[1] Natl Inst Adv Ind Sci & Technol, Age Dimens Res Ctr, Tsukuba, Ibaraki 3058562, Japan

[2] Tokyo Med & Dent Univ, Dept Orthopaed Surg, Bunkyo Ku, Tokyo 1138519, Japan

[3] JST, CREST, Shanghai, Peoples R China

[4] Fudan Univ, Zhongshan Hosp, Dept Orthopaed Surg, Shanghai 200032, Peoples R China

[5] Hlth Sci Univ Hokkaido, Sch Dent, Dept Operat Dent & Endodontol, Ishikari, Hokkaido 0610293, Japan

[6] Univ Tsukuba, Tsukuba, Ibaraki 3058575, Japan

[7] Natl Inst Mat Sci, Ctr Biomat, Tsukuba, Ibaraki 3050044, Japan

[8] AIST, Tissue Engn Res Ctr, Tsukuba, Ibaraki 3058562, Japan

[9] Univ Tokyo, Grad Sch Engn, Tokyo 1138656, Japan

来源：

BIOMATERIALS | 2003年 / 24卷 / 13期

关键词：

bone; regeneration; hydroxyapatite; beta-TCP; low-pressure; perfusion culture;

D O I：

10.1016/S0142-9612(03)00039-5

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The transplantation of cultured bone cells is expected to become a candidate for bone regeneration therapy. For the clinical application of this therapy, there remain several problems to be overcome, for example, the improvements of scaffolds and culture techniques. In this review article, two kinds of porous ceramics, a novel sintered porous hydroxyapatite and a porous beta-tricalcium phosphate (TCP), as well as a collagen-phosphosphoryn sponge are introduced as new scaffolds for bone regeneration. The former two ceramic scaffolds proved to be applicable for bone regeneration therapy. The collagen-phosphophoryn sponge proved to have bone formation ability in vivo. Moreover, for the application of this therapy to the regeneration of large bone defects, we improved the culture method by applying a low-pressure system and a perfusion system. Both culture systems accelerated the formation of bone in vivo in this transplantation model. Combinations of the scaffolds and culture techniques might be considered when designing therapeutic strategies. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：2277 / 2286

页数：10