Combining Information of Common Metabolites Reveals Global Differences between Colorectal Cancerous and Normal Tissues

被引：16

作者：

Chae, Young Kee ^{[1
,2
]}

Kang, Woo-Young ^{[1
,2
]}

Kim, Seong Hwan ^{[3
]}

Joo, Jong Eun ^{[4
]}

Han, Joon Kil ^{[5
]}

Hong, Boo Whan ^{[6
]}

机构：

[1] Sejong Univ, Dept Chem, Seoul 143747, South Korea

[2] Sejong Univ, Inst Biol Chem, Seoul 143747, South Korea

[3] Eulji Univ, Coll Med, Dept Internal Med, Seoul 139872, South Korea

[4] Eulji Univ, Coll Med, Dept Pathol, Seoul 139872, South Korea

[5] Eulji Univ, Coll Med, Dept Surg, Seoul 139872, South Korea

[6] Gwangju Samsung Hosp, Dept Surg, Kyonggi Do 464801, South Korea

来源：

BULLETIN OF THE KOREAN CHEMICAL SOCIETY | 2010年 / 31卷 / 02期

关键词：

NMR; Metabolomics; Profiling; Colorectal cancer; PCA; SPECTROSCOPY; METABONOMICS; TUMORS;

D O I：

10.5012/bkcs.2010.31.02.379

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Metabolites of colorectal cancer tissues from 12 patients were analyzed and compared with those of the normal tissues by two-dimensional NMR spectroscopy. NMR data were analyzed with the help of the metabolome database and the statistics software. Cancerous tissues showed significantly altered metabolic profiles as compared to the normal tissues. Among such metabolites, the concentrations of taurine, glutamate, choline were notably increased in the cancerous tissues of most patients, and those of glucose, malate, and glycerol were decreased. Changes in individual metabolites varied significantly from patient to patient, but the combination of such changes could be used to distinguish cancerous tissues from normal ones, which could be done by PCA analysis. The traditional chemometric analysis was also performed using AMIX software. By comparing those two results, the analysis via (1)H-(13)C HSQC spectra proved to be more robust and effective in assessing and classifying global metabolic profiles of the colorectal tissues.

引用

页码：379 / 383

页数：5

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