Pig cloning by microinjection of fetal fibroblast nuclei

被引：651

作者：

Onishi, A ^{[1
]}

Iwamoto, M

Akita, T

Mikawa, S

Takeda, K

Awata, T

Hanada, H

Perry, ACF

机构：

[1] Natl Inst Anim Ind, Dept Anim Breeding & Genet, Tsukuba Norin Danchi, Ibaraki 3050901, Japan

[2] Prima Meatpackers, Basic Res Div, Tsuchiura, Ibaraki 3000841, Japan

[3] Rockefeller Univ, Dev Biol Lab, New York, NY 10021 USA

来源：

SCIENCE | 2000年 / 289卷 / 5482期

关键词：

D O I：

10.1126/science.289.5482.1188

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Pig cloning will have a marked impact on the optimization of meat production and xenotransplantation. To clone pigs from differentiated cells, we microinjected the nuclei of porcine (Sus scrofa) fetal fibroblasts into enucleated oocytes, and development was induced by electroactivation. The transfer of 110 cloned embryos to four surrogate mothers produced an apparently normal female piglet. The clonal provenance of the piglet was indicated by her coat color and confirmed by DNA microsatellite analysis.

引用

页码：1188 / 1190

页数：3

共 25 条

[1] AMPLIFICATION OF THE ZFY AND ZFX GENES FOR SEX IDENTIFICATION IN HUMANS, CATTLE, SHEEP AND GOATS [J].

AASEN, E ;

MEDRANO, JF .

BIO-TECHNOLOGY, 1990, 8 (12) :1279-1281

[2] Production of goats by somatic cell nuclear transfer [J].

Baguisi, A ;

Behboodi, E ;

Melican, DT ;

Pollock, JS ;

Destrempes, MM ;

Cammuso, C ;

Williams, JL ;

Nims, SD ;

Porter, CA ;

Midura, P ;

Palacios, MJ ;

Ayres, SL ;

Denniston, RS ;

Hayes, ML ;

Ziomek, CA ;

Meade, HM ;

Godke, RA ;

Gavin, WG ;

Overström, EW ;

Echelard, Y .

NATURE BIOTECHNOLOGY, 1999, 17 (05) :456-461

[3]

Campbell K H, 1996, Rev Reprod, V1, P40, DOI 10.1530/ror.0.0010040

[4] Sheep cloned by nuclear transfer from a cultured cell line [J].

Campbell, KHS ;

McWhir, J ;

Ritchie, WA ;

Wilmut, I .

NATURE, 1996, 380 (6569) :64-66

[5] Cloned transgenic calves produced from nonquiescent fetal fibroblasts [J].

Cibelli, JB ;

Stice, SL ;

Golueke, PJ ;

Kane, JJ ;

Jerry, J ;

Blackwell, C ;

de Leon, FAP ;

Robl, JM .

SCIENCE, 1998, 280 (5367) :1256-1258

[6] Development of a culture medium (BECM-3) for porcine embryos: Effects of bovine serum albumin and fetal bovine serum on embryo development [J].

Dobrinsky, JR ;

Johnson, LA ;

Rath, D .

BIOLOGY OF REPRODUCTION, 1996, 55 (05) :1069-1074

[7] Preincubation of cumulus-oocyte complexes before exposure to gonadotropins improves the developmental competence of porcine embryos matured and fertilized in vitro [J].

Funahashi, H ;

Cantley, TC ;

Day, BN .

THERIOGENOLOGY, 1997, 47 (03) :679-686

[8] Clinical and pathologic features of cloned transgenic calves and fetuses (13 case studies) [J].

Hill, JR ;

Roussel, AJ ;

Cibelli, JB ;

Edwards, JF ;

Hooper, NL ;

Miller, MW ;

Thompson, JA ;

Looney, CR ;

Westhusin, ME ;

Robl, JM ;

Stice, SL .

THERIOGENOLOGY, 1999, 51 (08) :1451-1465

[9] Eight calves cloned from somatic cells of a single adult [J].

Kato, Y ;

Tani, T ;

Sotomaru, Y ;

Kurokawa, K ;

Kato, JY ;

Doguchi, H ;

Yasue, H ;

Tsunoda, Y .

SCIENCE, 1998, 282 (5396) :2095-2098

[10] Development of early porcine embryos in vitro and in vivo [J].

Macháty, Z ;

Day, BN ;

Prather, RS .

BIOLOGY OF REPRODUCTION, 1998, 59 (02) :451-455

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