Integrated chemical/biochemical sample collection, pre-concentration, and analysis on a digital microfluidic lab-on-a-chip platform

被引:41
作者
Fair, RB [1 ]
Khlystov, A [1 ]
Srinivasan, V [1 ]
Pamula, VK [1 ]
Weaver, KN [1 ]
机构
[1] Duke Univ, Dept Elect & Comp Engn, Durham, NC 27708 USA
来源
LAB - ON - A - CHIP: PLATFORMS, DEVICES, AND APPLICATIONS | 2004年 / 5591卷
关键词
lab-on-a-chip; digital microfluidics; sample collection; detection; analysis;
D O I
10.1117/12.581955
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
An ideal on-site chemical/biochemical analysis system must be inexpensive, sensitive, fully automated and integrated. reliable, and compatible with a broad range of samples. The advent of digital microfluidic lab-on-a-chip (LoC) technology offers such a detection system due to the advantages in portability, reduction of the volumes of the sample and reagents, faster analysis times, increased automation, low power consumption, compatibility with mass manufacturing, and high throughput. We describe progress towards integrating sample collection onto a digital microfluidic LoC that is a component of a cascade impactor device. The sample collection is per-formed by impacting airborne particles directly onto the surface of the chip. After the collection phase, the surface of the chip is washed with a micro-droplet of solvent. The droplet will be, digitally directed across the impaction surface, dissolving sample constituents. Because of the very small droplet volume used for extraction of the sample from a wide collection area. the resulting solution is relatively concentrated and the analytes can be detected after a very short sampling time (1 min) due to such pre-concentration. After the washing-phase, the droplet is mixed with specific reagents that produce colored reaction products. The concentration of the analyte is quantitatively determined by measuring absorption at target wavelengths using a simple light emitting diode and photodiode setup. Specific applications include automatic measurements of major inorganic ions in aerosols, such as sulfate, nitrate and ammonium, with a time resolution of 1 min and a detection limit of 30 ng/m(3). We have already demonstrated the detection and quantification of nitroaromatic explosives without integrating the sample collection. Other applications being developed include airborne bioagent detection.
引用
收藏
页码:113 / 124
页数:12
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