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Study of mixed Langmuir-Blodgett films of immunoglobulin G/amphiphile and their application for immunosensor engineering

被引：21
作者：
Hou, YX
Tlili, C
Jaffrezic-Renault, N
Zhang, AD
Martelet, C
Ponsonnet, L
Errachid, A
Samitier, J
Bausells, J
机构：
[1] Ecole Cent Lyon, IFOS, CNRS, UMR 5621, F-69131 Ecully, France
[2] Cent China Normal Univ, Coll Chem, Wuhan 430079, Peoples R China
[3] FSc Monastir, LPCI, Monastir 5000, Tunisia
[4] Lab Nanobioengn, Barcelona 08028, Spain
[5] Ctr Nacl Microelect IMB CSIC, Barcelona 08193, Spain
来源：
BIOSENSORS & BIOELECTRONICS | 2004年 / 20卷 / 06期
关键词：
LB films; immunoglobulin G; octadecylamine; behenic acid; ac impedance; immunosensors;
D O I：
10.1016/j.bios.2004.05.017
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Langmuir-Blodgett (LB) technique appears to be quite suitable for generating biospecific surfaces and it has potential application for fabricating biosensors. In this work, mixed Langmuir-Blodgett films of immunoglobulin G/amphiphile have been transferred onto hydrophobic silver surface previously modified by I-octadecanethiol (ODT) SAMs. In order to obtain stable LB films, the influences of different parameters - type of amphiphile, surface pressure and pH - on the properties of mixed IgG/amphiphile monolayer. were investigated. Electrochemical properties of the engineered immunosensor have been measured by impedimetric spectroscopy. The immunosensor obtained exhibits a high sensitivity and a good specificity in a linear dynamic range from 200 to 1000 ng ml(-1). (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：1126 / 1133
页数：8
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