Diagnostic Approach for the Differentiation of the Pandemic Influenza A(H1N1)v Virus from Recent Human Influenza Viruses by Real-Time PCR

被引：63

作者：

Schulze, Martin ^{[1
]}

Nitsche, Andreas ^{[2
]}

Schweiger, Brunhilde ^{[1
]}

Biere, Barbara ^{[1
]}

机构：

[1] Fachgebiet 17 Influenza Respirator Viren, Robert Koch Inst, Berlin, Germany

[2] Zentrum Biol Sicherheit 1, Robert Koch Inst, Berlin, Germany

来源：

PLOS ONE | 2010年 / 5卷 / 03期

关键词：

H1N1; 1ST;

D O I：

10.1371/journal.pone.0009966

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Background: The current spread of pandemic influenza A(H1N1)v virus necessitates an intensified surveillance of influenza virus infections worldwide. So far, in many laboratories routine diagnostics were limited to generic influenza virus detection only. To provide interested laboratories with real-time PCR assays for type and subtype identification, we present a bundle of PCR assays with which any human influenza A and B virus can be easily identified, including assays for the detection of the pandemic A(H1N1)v virus. Principal Findings: The assays show optimal performance characteristics in their validation on plasmids containing the respective assay target sequences. All assays have furthermore been applied to several thousand clinical samples since 2007 (assays for seasonal influenza) and April 2009 (pandemic influenza assays), respectively, and showed excellent results also on clinical material. Conclusions: We consider the presented assays to be well suited for the detection and subtyping of circulating influenza viruses.

引用

页数：6