Branched oligonucleotides containing bicyclic nucleotides as branching points and DNA or LNA as triples forming branch

被引：17

作者：

Sorensen, MD ^{[1
]}

Meldgaard, M ^{[1
]}

Rajwanshi, VK ^{[1
]}

Wengel, J ^{[1
]}

机构：

[1] Univ Copenhagen, Dept Chem, Ctr Synthet Bioorgan Chem, DK-1168 Copenhagen, Denmark

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2000年 / 10卷 / 16期

关键词：

D O I：

10.1016/S0960-894X(00)00362-0

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Various Y-shaped branched oligonucleotides containing a 2'-O,3'-C-ethylene linked or 2'-O,4'-C-methylene linked bicyclic nucleotide as branching point were synthesized on an automated DNA synthesizer. Thermal denaturation experiments at 260 and 284 nm showed increased thermal stabilities of complexes formed between these Y-shaped oligonucleotides and complementary DNA compared with those formed with the corresponding linear reference. The most significant effect was observed when LNA (locked nucleic acid) monomers were used in the tripler forming branch. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：1853 / 1856

页数：4

共 24 条

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