Up-regulation of genes encoding glycosylphosphatidylinositol (GPI)-attached proteins in response to cell wall damage caused by disruption of FKS1 in Saccharomyces cerevisiae

被引：72

作者：

Terashima, H ^{[1
]}

Yabuki, N ^{[1
]}

Arisawa, M ^{[1
]}

Hamada, K ^{[1
]}

Kitada, K ^{[1
]}

机构：

[1] Nippon Roche Res Ctr, Dept Mycol, Kanagawa 2478530, Japan

来源：

MOLECULAR AND GENERAL GENETICS | 2000年 / 264卷 / 1-2期

关键词：

FKS1; cell wall damage; RLM1; GPI proteins; Saccharomyces cerevisiae;

D O I：

10.1007/s004380000285

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

FKS1 and FKS2 encode alternative catalytic subunits of the glucan synthases that are responsible for synthesis of beta-1,3-glucan in the Saccharomyces cerevisiae cell wall. Disruption of FKS1 reduces the glucan content of the cell wall. increases chitin content and activates the expression of CWP1, which encodes a glycosylphosphatidylinositol (GPI)-dependent cell wall protein. These cellular responses have been regarded as compensating for cell wall damage in order to maintain cell wall integrity. Here. we report the identification, by genome-wide screening, of 22 genes that are transcriptionally up-regulated in fks1 Delta cells. Among them, five genes were found to encode GPI-attached proteins, three of which are covalently associated with the cell wall. Deletion and replacement analysis of the promoter regions identified Rlm1-binding sequences as being responsible for the up-regulation following disruption of FKS1. Using the rlm1 Delta tetOp-FKS1 strain, in which the expression of FKS1 can be repressed by doxycycline, we examined the requirement for Rlm1 for the transcriptional up-regulation of these five genes. Three of the five genes were not up-regulated by doxycycline, indicating that Rlm1 mediates their up-regulation when FKS1 is inactivated. The remaining two genes were up-regulated by doxycycline, suggesting that a transcription factor other than Rlm1 is involved in their response to disruption of FKS1.

引用

页码：64 / 74

页数：11

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