Role of mast cells in the onset of IgE-mediated late-phase cutaneous response in mice

被引:20
作者
Nagai, H [1 ]
Abe, T [1 ]
Yamaguchi, I [1 ]
Mito, K [1 ]
Tsunematsu, M [1 ]
Kimata, M [1 ]
Inagaki, N [1 ]
机构
[1] Gifu Pharmaceut Univ, Dept Pharmacol, Gifu 5028585, Japan
关键词
mast cells; degranulation;
D O I
10.1067/mai.2000.106778
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background: In mice that are passively sensitized to IgE, cutaneous antigen challenge produces a biphasic response with peaks at 1 and 24 hours after challenge. Objective: We investigated the role of mast cells in the IgE-mediated late-phase reaction in mice. Methods: We histologically and ultrastructurally investigated the morphologic changes of mast cells during the biphasic responses. Results: Degranulation of mast cells, which was observed between 4 and 24 hours after challenge, reached a peak at 8 hours. Piecemeal degranulation was seen during the immediate phase reaction. The number of IL-6-positive mast cells was increased after 4 hours in both IgE-sensitized and unsensitized mice, but positive cells showed a greater increase in sensitized mice and reached a peak after 8 hours. With in situ hybridization experiments, mast cells were positive for IL-6 messenger RNA at 6 hours after challenge. Conclusion: These findings indicate that anaphylactic degranulation of mast cells and the expression of IL-6 mRNA within 4 hours after antigen challenge are important for the onset of the late-phase allergic cutaneous reaction in mice.
引用
收藏
页码:S91 / S98
页数:8
相关论文
共 30 条
[21]   PASSIVE CUTANEOUS ANAPHYLAXIS IN MOUSE SKIN IS ASSOCIATED WITH LOCAL ACCUMULATION OF INTERLEUKIN-6 MESSENGER-RNA AND IMMUNOREACTIVE INTERLEUKIN-6 PROTEIN [J].
MICAN, JAM ;
ARORA, N ;
BURD, PR ;
METCALFE, DD .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1992, 90 (05) :815-824
[22]  
NAGAI H, 1995, BIOL PHARM BULL, V18, P239
[23]   TNF-alpha participates in an IgE-mediated cutaneous reaction in mast cell deficient, WBB6F1-W/W-V mice [J].
Nagai, H ;
Sakurai, T ;
Abe, T ;
Matsuo, A ;
Musoh, K ;
Tsunematsu, M ;
Inagaki, N .
INFLAMMATION RESEARCH, 1996, 45 (03) :136-140
[24]  
OXHOLM A, 1992, APMIS S, V24, P5
[25]   LATE-PHASE REACTION IN ASTHMA - BASIC MECHANISMS [J].
PRADALIER, A .
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 1993, 101 (03) :322-325
[26]  
RAY A, 1989, ANN NY ACAD SCI, V557, P353
[27]  
Sawada K, 1997, CLIN EXP ALLERGY, V27, P225
[28]   RECRUITMENT OF NEUTROPHILS DURING IGE-DEPENDENT CUTANEOUS LATE PHASE REACTIONS IN THE MOUSE IS MAST CELL-DEPENDENT - PARTIAL INHIBITION OF THE REACTION WITH ANTISERUM AGAINST TUMOR-NECROSIS-FACTOR-ALPHA [J].
WERSHIL, BK ;
WANG, ZS ;
GORDON, JR ;
GALLI, SJ .
JOURNAL OF CLINICAL INVESTIGATION, 1991, 87 (02) :446-453
[29]  
WERSHIL BK, 1995, J IMMUNOL, V154, P1391
[30]  
WILLIAMS CMM, 1995, IMMUNOLOGY, V86, P244