Strain improvement of Lactobacillus lactis for d-lactic acid production

被引：59

作者：

Joshi, D. S. ^{[1
]}

Singhvi, M. S. ^{[1
]}

Khire, J. M. ^{[1
]}

Gokhale, D. V. ^{[1
]}

机构：

[1] Natl Chem Lab, NCIM Resource Ctr, Pune 411008, Maharashtra, India

来源：

BIOTECHNOLOGY LETTERS | 2010年 / 32卷 / 04期

关键词：

Cellobiose utilization; Lactobacillus lactis; D-Lactic acid production; Mutant; Sucrose (cane sugar); CORYNEBACTERIUM-GLUTAMICUM; DELBRUECKII; FERMENTATION; CELLOBIOSE; RICE;

D O I：

10.1007/s10529-009-0187-y

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Three mutants, isolated by repeated UV mutagenesis of Lactobacillus lactis NCIM 2368, produced increased d-lactic acid concentrations. These mutants were compared with the wild type using 100 g hydrolyzed cane sugar/l in the fermentation medium. One mutant, RM2-24, produced 81 g lactic acid/l which was over three times that of the wild type. The highest d-lactic acid (110 g/l) in batch fermentation was obtained with 150 g cane sugar/l with a 73% lactic acid yield. The mutant utilizes cellobiose efficiently, converting it into d-lactic acid suggesting the presence of cellobiase. Thus, this strain could be used to obtain d-lactic acid from cellulosic materials that are pre-hydrolyzed with cellulase.

引用

页码：517 / 520

页数：4

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