Reduced pyrene bioavailability in microbially active soils

被引:94
作者
Guthrie, EA [1 ]
Pfaender, FK [1 ]
机构
[1] Univ N Carolina, Sch Publ Hlth, Dept Environm Sci & Engn, Chapel Hill, NC 27599 USA
关键词
D O I
10.1021/es970670d
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The influence of microbial activity on pyrene biodegradation and fate was assessed by quantifying the distribution of C-14-label and pyrene among soil organic matter fractions (SOM) in soils that contained varied microbial communities. A southern, hardwood forest soil was incubated with pyrene in aerated chambers for 9 months. Soil was incubated in three sets: soil alone, soil augmented with a pyrene-degrading community, and soil inhibited with sodium azide (NaN3). After 9 months, pyrene mineralization was observed only in the degrader-amended soil set. Most of the pyrene and [C-14]pyrene in the NaN3-amended soil set was recovered by solvent extraction (>75%). The [C-14]pyrene extracted from the NaN3-amended soil set was mineralized by a microbial community shown to mineralize pyrene. Extractable pyrene from nonamended soil and degrader-amended soil sets decreased with time due to biodegradation. Extracted label from these soils was not mineralized to the same extent as NaN3-amended soil. Nonextractable label in humic/fulvic acid and humin fractions was greater in these soils than in the NaN3-amended soil set. Pyrene degradation products were detected in biologically active soils by HPLC. Over time, the extractability of pyrene and pyrene products in biologically active soils decreased to a greater extent than NaN3-amended soil.
引用
收藏
页码:501 / 508
页数:8
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