A novel toxin homologous to large clostridial cytotoxins found in culture supernatant of Clostridium perfringens type C

被引:102
作者
Amimoto, Katsuhiko [1 ]
Noro, Taichi [1 ]
Oishi, Eiji [1 ]
Shimizu, Mitsugu [1 ]
机构
[1] Kyoto Biken Labs Inc, Uji, Kyoto 6110041, Japan
来源
MICROBIOLOGY-SGM | 2007年 / 153卷
关键词
D O I
10.1099/mic.0.2006/002287-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
An unknown cytotoxin was identified in the culture supernatant of Clostridium perfringens type C. The cytotoxin, named TpeL, which was purified using mAb-based affinity chromatography, had a lethal activity of 62 minimum lethal dose (MLD) mg(-1) in mice and a cytotoxic activity of 6.2 x 10(5) cytotoxic units (CU) mg(-1) in Vero cells. The nucleotide sequence of TpeL was determined. The entire ORF had a length of 4953 bases, and the same.nucleotide sequence was not recorded in the GenBank/EMBL/DDBJ databases. The molecular mass calculated from the deduced amino acid sequence was 191 kDa, and a signal peptide region was not found within the ORF. The deduced amino acid sequence exhibited 30-39 % homology to Clostridium difficile toxins A (TcdA) and B (TcdB), Clostridium sordellii lethal toxin (TcsL) and Clostridium novyi alpha-toxin (TcnA). The amino acid sequence of TpeL is shorter than these toxins, and the homologous region was located at the N-terminal site. Eighteen strains of C. perfringens types A, B and C were surveyed for the presence of the tpeL gene by PCR. The tpeL gene was detected in all type B (one strain) and C strains (five strains), but not in any type A strains (12 strains). TpeL was detected in culture filtrates of the five type C strains by dot-blot analysis, but not in the type B strain. It was concluded that TpeL is a novel toxin similar to the known large clostridial cytotoxins. Furthermore, the data indicated that TpeL is produced by many C. perfringens type C strains.
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页码:1198 / 1206
页数:9
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