Stimulated serotonin release from hyperinnervated terminals subsequent to neonatal dopamine depletion regulates striatal tachykinin, but not enkephalin gene expression

被引:14
作者
Basura, GJ
Walker, PD
机构
[1] Wayne State Univ, Sch Med, Dept Anat & Cell Biol, Detroit, MI 48201 USA
[2] Wayne State Univ, Sch Med, Cellular & Clin Neurobiol Program, Dept Psychiat & Behav Neurosci, Detroit, MI 48201 USA
来源
MOLECULAR BRAIN RESEARCH | 2000年 / 81卷 / 1-2期
关键词
6-hydroxydopamine; basal ganglia; p-chloroamphetamine; preprotachykinin; preproenkephalin; Parkinson's disease;
D O I
10.1016/S0169-328X(00)00153-4
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Dopamine (DA) depletion in neonatal rodents results in depressed tachykinin and elevated enkephalin gene expression in the adult striatum (STR). Concurrently, serotonin (5-HT) fibers sprout to hyperinnervate the DA-depleted anterior striatum (A-STR). The present study was designed to determine if increased 5-HT release from sprouted terminals influences dysregulated preprotachykinin (PPT) and preproenkephalin (PPE) mRNA expression in the DA-depleted STR. Three-day-old Sprague-Dawley rat pups received bilateral intracerebroventricular injections of vehicle or the DA neurotoxin 6-hydroxydopamine (6-OHDA, 100 mu g). Two months later, rats received a single intraperitoneal injection of vehicle or the acute 5-HT releasing agent p-chloroamphetamine (PCA; 10 mg/kg). Rats were killed 4 h later and striata processed for monoamine content by HPLC-ED and mRNA expression by in situ hybridization within specific subregions of the A-STR and posterior striatum (P-STR). 6-OHDA treatment severely (>98%) reduced striatal DB levels, while 5-HT content in the A-STR was significantly elevated (doubled), indicative of 5-HT hyperinnervation. Following 6-OHDA, PPT mRNA levels were depressed 60-66% across three subregions of the A-STR and 52-59% across two subregions of the P-STR, while PPE mRNA expression was elevated in both the A-STR (50-62%) and P-STR (55-82%). PCA normalized PPT mRNA levels in all regions of the DA-depleted A-STR and P-STR, yet did not alter PPE levels in either dorsal central or medial regions from 6-OHDA alone, but reduced PPE to control levels in the dorsal lateral A-STR. These data indicate that increased 5-HT neurotransmission, following neonatal 6-OHDA treatment, primarily influences PPT-containing neurons of the direct striatal output pathway. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:80 / 91
页数:12
相关论文
共 60 条
[1]   THE FUNCTIONAL-ANATOMY OF BASAL GANGLIA DISORDERS [J].
ALBIN, RL ;
YOUNG, AB ;
PENNEY, JB .
TRENDS IN NEUROSCIENCES, 1989, 12 (10) :366-375
[2]   I-125 LSD AUTORADIOGRAPHY CONFIRMS THE PREFERENTIAL LOCALIZATION OF CAUDATE-PUTAMEN-S2-RECEPTORS TO THE CAUDAL (PERIPALLIDAL) REGION [J].
ALTAR, CA ;
BOYAR, WC ;
MARIEN, MR .
BRAIN RESEARCH, 1986, 372 (01) :130-136
[3]   EXTENSIVE COOCCURRENCE OF SUBSTANCE-P AND DYNORPHIN IN STRIATAL PROJECTION NEURONS - AN EVOLUTIONARILY CONSERVED FEATURE OF BASAL GANGLIA ORGANIZATION [J].
ANDERSON, KD ;
REINER, A .
JOURNAL OF COMPARATIVE NEUROLOGY, 1990, 295 (03) :339-369
[4]   STRIATAL TACHYKININ BIOSYNTHESIS - REGULATION OF MESSENGER-RNA AND PEPTIDE LEVELS BY DOPAMINE AGONISTS AND ANTAGONISTS [J].
BANNON, MJ ;
ELLIOTT, PJ ;
BUNNEY, EB .
MOLECULAR BRAIN RESEARCH, 1987, 3 (01) :31-37
[5]   MEDIAL FOREBRAIN-BUNDLE STIMULATION OR D-2 DOPAMINE RECEPTOR ACTIVATION INCREASES PREPROENKEPHALIN MESSENGER-RNA IN RAT STRIATUM [J].
BANNON, MJ ;
KELLAND, M ;
CHIODO, LA .
JOURNAL OF NEUROCHEMISTRY, 1989, 52 (03) :859-862
[6]   NEUROKININ-3 RECEPTORS MODULATE DOPAMINE CELL-FUNCTION AND ALTER THE EFFECTS OF 6-HYDROXYDOPAMINE [J].
BANNON, MJ ;
BROWNSCHIDLE, LA ;
TIAN, Y ;
WHITTY, CJ ;
POOSCH, MS ;
DSA, C ;
MOODY, CA .
BRAIN RESEARCH, 1995, 695 (01) :19-24
[7]   SUBSTANCE-P AND NEUROKININ-A REGULATE BY DIFFERENT MECHANISMS DOPAMINE RELEASE FROM DENDRITES AND NERVE-TERMINALS OF THE NIGROSTRIATAL DOPAMINERGIC-NEURONS [J].
BARUCH, P ;
ARTAUD, F ;
GODEHEU, G ;
BARBEITO, L ;
GLOWINSKI, J ;
CHERAMY, A .
NEUROSCIENCE, 1988, 25 (03) :889-898
[8]   Serotonin 2A receptor mRNA levels in the neonatal dopamine-depleted rat striatum remain upregulated following suppression of serotonin hyperinnervation [J].
Basura, GJ ;
Walker, PD .
DEVELOPMENTAL BRAIN RESEARCH, 1999, 116 (01) :111-117
[9]   Differential sensitivity of tachykinin vs. enkephalin gene expression in the posterior striatum in response to acute p-chloroamphetamine treatment during postnatal development [J].
Basura, GJ ;
Walker, PD .
DEVELOPMENTAL BRAIN RESEARCH, 1999, 112 (01) :155-157
[10]   Suppression of serotonin hyperinnervation does not alter the dysregulatory influences of dopamine depletion on striatal neuropeptide gene expression in rodent neonates [J].
Basura, GJ ;
Walker, PD .
NEUROSCIENCE LETTERS, 1999, 274 (01) :9-12