Rapid solid-phase extraction method for automated gas chromatographic mass spectrometric determination of nicotine in plasma

被引：15

作者：

Davoli, E ^{[1
]}

Stramare, L ^{[1
]}

Fanelli, R ^{[1
]}

Diomede, L ^{[1
]}

Salmona, M ^{[1
]}

机构：

[1] Mario Negri Inst Pharmacol Res, I-20157 Milan, Italy

来源：

JOURNAL OF CHROMATOGRAPHY B | 1998年 / 707卷 / 1-2期

关键词：

nicotine;

D O I：

10.1016/S0378-4347(97)00579-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study was to develop a simple, rapid and sensitive assay of nicotine in plasma for automated gas chromatographic-mass spectrometric analysis. Biological samples were extracted using pre-packed Extrelut-1 columns with 5 ml of ethyl acetate. Quantitative analysis was done using deuterium-labelled nicotine as internal standard. The limit of quantitation was 0.5 ng in 1-ml plasma samples. Precision was ranging from 13.3% to 1.64% (R.S.D.) depending on the concentration, while the deviation was 4.16%. This method has been used for determination of nicotine bioavailability from new, low-dosage, nicotine chewing gum strips. (C) 1998 Elsevier Science B.V.

引用

页码：312 / 316

页数：5

共 8 条

[1]

DAVIS RA, 1986, J CHROMATOGR SCI, V24, P134, DOI 10.1093/chromsci/24.4.134

[2] MASS SPECTROMETRY IN STRUCTURAL AND STEREOCHEMICAL PROBLEMS .72. A STUDY OF FRAGMENTATION PROCESSES OF SOME TOBACCO ALKALOIDS [J].