The determination of creatinine in human urine by capillary zone electrophoresis with photodiode array detection

被引:6
作者
Jia, L [1 ]
Gao, J [1 ]
Chen, X [1 ]
Hu, GL [1 ]
Wang, YR [1 ]
Wang, XR [1 ]
Zhuang, ZX [1 ]
机构
[1] Xiamen Univ, Dept Chem, Res Lab SEDC Analyt Sci Mat & Life Chem, Xiamen 361005, Peoples R China
基金
中国国家自然科学基金; 中国博士后科学基金;
关键词
D O I
10.1080/10826079808005862
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The application of capillary zone electrophoresis (CZE) to the separation and determination of creatinine in human urine with photodiode array detection was described. Optimum conditions for the separation were established. The effect of pH of the background buffer on the existence state of creatinine was investigated. Photodiode array detection permitted the rapid identification of creatinine in the samples analysed. By selecting 234 Mn as monitoring wavelength, the interference from urea in urine was eliminated. By selecting a suitable pH, the interferences from other substances in urine were also eliminated. The linear range, detection limit, precision and recovery of the method were investigated.
引用
收藏
页码:965 / 977
页数:13
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