HPLC/DAD analysis of phenolic compounds from lavender and its application to quality control

被引：45

作者：

Areias, FM ^{[1
]}

Valentao, P ^{[1
]}

Andrade, PB ^{[1
]}

Moreira, MM ^{[1
]}

Amaral, J ^{[1
]}

Seabra, RM ^{[1
]}

机构：

[1] Univ Porto, Fac Farm, CEQUP, Lab Farmacognosia, P-4050 Porto, Portugal

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2000年 / 23卷 / 16期

关键词：

D O I：

10.1081/JLC-100100510

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reversed phase HPLC procedure is proposed for the determination of eight phenolic compounds (2-O-glucosilcoumaric acid, o-coumaric acid, rosmarinic acid, apigenin-7-O-glucoside, coumarin, herniarin, luteolin, and apigenin) in lavender. The chromatographic separation was achieved using a reversed-phase Spherisorb ODS 2 (5 mu m particle size: 25.0 x 0.46 cm) column. From the several extractive solvents assayed, only ethanol was able to extract all the mentioned compounds. Best resolution was obtained using a gradient of water-formic acid (19:1) and acetonitrile. Ten samples were subjected to quantification, all of them showing a common composition pattern.

引用

页码：2563 / 2572

页数：10

共 9 条

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