Restricted expression of membrane type 1-matrix metalloproteinase by myofibroblasts adjacent to human breast cancer cells

被引:55
作者
Bisson, C
Blacher, S
Polette, M
Blanc, JF
Kebers, F
Desreux, J
Tetu, B
Rosenbaum, J
Foidart, JM
Birembaut, P
Noel, A [1 ]
机构
[1] Univ Liege, Lab Tumor & Dev Biol, B-4000 Liege, Belgium
[2] Univ Bordeaux 2, INSERM E0362, Grp Rech Etud Foie, F-33076 Bordeaux, France
[3] Univ Bordeaux 2, Federat Res Inst, IFR66, F-33076 Bordeaux, France
[4] CHU Maison Blanche, INSERM, U514, Lab Pol Bouin, Reims, France
[5] Hop Hotel Dieu, Dept Pathol, Quebec City, PQ, Canada
关键词
MTI-MMP; myofibroblast; cancer; stroma; alpha smooth muscle actin; MMP-2;
D O I
10.1002/ijc.11012
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The membrane type-1 matrix metalloproteinase (MT1-MMP), a protease originally identified in breast carcinoma, is characterized by its capacity to Activate other MMPs (MMP-2 and MMP-13) and to degrade extracellular matrix. Our study was undertaken to localize and identify the MT1-MMP expressing cells in human breast adenocarcinomas. A textural analysis of images obtained by immunohistochemistry and in situ hybridization showed precisely the co-expression of alpha smooth muscle actin (alphaSM actin) and MT1-MMP in myofibroblasts. MT1-MMP expression is confined to myofibroblasts in close contact with tumor cells. In sharp contrast, the expression of MMP-2 was more widely distributed in both aSM actin positive and negative cells close to and at distance from cancer cell clusters. Our in vitro observations are consistent with the higher level of MT1-MMP expression and of MMP-2 activation observed in alphaSM actin positive fibroblasts derived from breast tumors, at compared to normal breast fibroblasts. Collectively, these results implicate myofibroblasts as major producer of MT1-MMP in breast cancer and emphasize the importance of stromal-epithelial cell interactions in their progression. (C) 2003 Wiley-Liss, Inc.
引用
收藏
页码:7 / 13
页数:7
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