A novel β1,3-N-acetylglucosaminyltransferase (β3Gn-T8), which synthesizes poly-N-acetyllactosamine, is dramatically upregulated in colon cancer

被引：78

作者：

Ishida, H

Togayachi, A

Sakai, T

Iwai, T

Hiruma, T

Sato, T

Okubo, R

Inaba, N

Kudo, T

Gotoh, M

Shoda, J

Tanaka, N

Narimatsu, H

机构：

[1] Natl Inst Adv Ind Sci & Technol AIST, Res Ctr Glycosci, Glycogene Funct Team, Tsukuba, Ibaraki 3068568, Japan

[2] Univ Tsukuba, Inst Clin Med, Dept Gastroenterol, Tsukuba, Ibaraki 305, Japan

[3] Seikagaku Corp, Cent Res Labs, Higashiyamato, Tokyo 2070021, Japan

[4] Fujirebio Inc, Fundamental Res Dept, Frontier Res Div, Hachioji, Tokyo 1920031, Japan

[5] Yokohama Natl Univ, Grad Sch Environm & Informat Sci, Hodogaya Ku, Yokohama, Kanagawa 2408501, Japan

[6] JGS Japan Genome Solut Inc, Hachiaji, Tokyo 1920031, Japan

[7] Univ Tsukuba, Inst Basic Med Sci, Dept Anat & Embryol, Tsukuba, Ibaraki 305, Japan

来源：

FEBS LETTERS | 2005年 / 579卷 / 01期

关键词：

glycosyltransferase; beta; 1.3-N-acetylglucosaminyl-transferase; polylactosamine; colon cancer; N-glycan;

D O I：

10.1016/j.febslet.2004.11.037

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new member of the UDP-N-acetvlalucosamine: beta galactose beta1,3-N-acetyliglucosaminyltransferase (beta3Gn-T) family having the beta3-glycosyltransferase motifs was identified using an in silico method. This novel beta3Gn-T was cloned from a human colon cancer cell line and named beta3Gn-T8 based on its position in a phylogenetic tree and enzymatic activity. beta3Gn-T8 transfers GlcNAc to the non-reducing terminus of the Galbeta1-4GlcNAc of tetraantennary N-glycan in vitro. HCT15 cells transfected with beta3Gn-T8 cDNA showed an increase in reactivity to both LEA and PHA-L4 in a flow cytometric analysis. These results indicated that beta3Gn-T8 is involved in the biosynthesis of poly-N-acetyllactosamine chains on tetraantennary (beta1,6-branched) N-glycan. In most of the colorectal cancer tissues examined, the level of beta3Gn-T8 transcript was significantly higher than in normal tissue. beta3Gn-T8 could be an enzyme involved in the synthesis of poly-N-acetyflactosamine on beta1-6 branched N-glycans in colon cancer. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：71 / 78

页数：8

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