Ultrahigh Sensitivity Carbon Nanotube Agents for Photoacoustic Molecular Imaging in Living Mice

被引：327

作者：

de la Zerda, Adam ^{[2
,3
,4
]}

Liu, Zhuang ^{[1
,6
]}

Bodapati, Sunil ^{[2
,3
]}

Teed, Robert ^{[2
,3
]}

Vaithilingam, Srikant ^{[4
]}

Khuri-Yakub, Butrus T. ^{[4
]}

Chen, Xiaoyuan ^{[2
,3
,7
,8
]}

Dai, Hongjie ^{[1
]}

Gambhir, Sanjiv Sam ^{[2
,3
,5
]}

机构：

[1] Stanford Univ, Dept Chem, Palo Alto, CA 94305 USA

[2] Stanford Univ, Dept Radiol, Mol Imaging Program Stanford, Palo Alto, CA 94305 USA

[3] Stanford Univ, Bio X Program, Palo Alto, CA 94305 USA

[4] Stanford Univ, Dept Elect Engn, Palo Alto, CA 94305 USA

[5] Stanford Univ, Dept Bioengn, Palo Alto, CA 94305 USA

[6] Soochow Univ, Funct Nano & Soft Mat Lab FUNSOM, Suzhou 215123, Jiangsu, Peoples R China

[7] Natl Inst Biomed Imaging & Bioengn, Lab Mol Imaging & Nanomed, Bethesda, MD 20892 USA

[8] Natl Inst Biomed Imaging & Bioengn, NIH, Bethesda, MD 20892 USA

来源：

NANO LETTERS | 2010年 / 10卷 / 06期

关键词：

Photoacoustic molecular imaging; carbon nanotube; IN-VIVO;

D O I：

10.1021/nl100890d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Photoacoustic imaging is an emerging modality that overcomes to a great extent the resolution and depth limitations of optical imaging while maintaining relatively high-contrast. However, since many diseases will not manifest an endogenous photoacoustic contrast, it is essential to develop exogenous photoacoustic contrast agents that can target diseased tissue(s). Here we present a novel photoacoustic contrast agent, Indocyanine Green dye-enhanced single walled carbon nanotube (SWNT-ICG). We conjugated this contrast agent with cyclic Arg-Gly-Asp (RGD) peptides to molecularly target the alpha(nu)beta(3) integrins, which are associated with tumor angiogenesis. Intravenous administration of this tumor-targeted contrast agent to tumor-bearing mice showed significantly higher photoacoustic signal in the tumor than in mice injected with the untargeted contrast agent. The new contrast agent gave a markedly 300 times higher photoacoustic contrast in living tissues than previously reported SWNTs, leading to subnanomolar sensitivities. Finally, we show that the new contrast agent can detect similar to 20 times fewer cancer cells than previously reported SWNTs.

引用

页码：2168 / 2172

页数：5

共 14 条

[1] Carbon nanotubes as photoacoustic molecular imaging agents in living mice [J].