Properties of glucose sensors based on the immobilization of glucose oxidase in N-substituted polypyrrole film

被引:37
作者
Yasuzawa, M [1 ]
Nieda, T [1 ]
Hirano, T [1 ]
Kunugi, A [1 ]
机构
[1] Univ Tokushima, Fac Engn, Dept Chem Sci & Technol, Tokushima 7708506, Japan
关键词
glucose sensors; electropolymerization; pyrrole derivatives; cross-linking;
D O I
10.1016/S0925-4005(99)00453-0
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Amperometoric glucose sensors were prepared by the electropolymerization of 3-(1-pyrrolyl)propionic acid (PPA) in the presence of the enzyme, following the treatment with water-soluble carbodiimide (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, EDC) to provide covalent bonding between glucose oxidase (GOD) and polypyrrole derivatives. Poly(o-phenylenediamine) (PPD) and Nafion films were introduced as the inner film in the same electrodes. The EDC treatment was effective to improve the stability of the glucose response except on the electrode with Nafion inner film. Though neither electrode was influenced by the addition of urea or D-(-)-fructose, the influence of ascorbic acid, acetaminophen and uric acid was not negligible even on the electrode with inner film. (C) 2000 Elsevier Science S.A. All rights reserved.
引用
收藏
页码:77 / 79
页数:3
相关论文
共 12 条
[1]   PULSED AMPEROMETRIC DETECTION OF GLUCOSE IN BIOLOGICAL-FLUIDS AT A SURFACE-MODIFIED GOLD ELECTRODE [J].
BINDRA, DS ;
WILSON, GS .
ANALYTICAL CHEMISTRY, 1989, 61 (22) :2566-2570
[2]  
FOULDS NC, 1986, J CHEM SOC F1, V82, P143
[3]   CHARACTERIZATION OF GLUCOSE OXIDASE-MODIFIED POLY(PHENYLENEDIAMINE)-COATED ELECTRODES IN-VITRO AND IN-VIVO - HOMOGENEOUS INTERFERENCE BY ASCORBIC-ACID IN HYDROGEN-PEROXIDE DETECTION [J].
LOWRY, JP ;
MCATEER, K ;
ELATRASH, SS ;
DUFF, A ;
ONEILL, RD .
ANALYTICAL CHEMISTRY, 1994, 66 (10) :1754-1761
[4]   PERFORMANCE OF SUBCUTANEOUSLY IMPLANTED NEEDLE-TYPE GLUCOSE SENSORS EMPLOYING A NOVEL TRILAYER COATING [J].
MOUSSY, F ;
HARRISON, DJ ;
OBRIEN, DW ;
RAJOTTE, RV .
ANALYTICAL CHEMISTRY, 1993, 65 (15) :2072-2077
[5]   A GLUCOSE MONITORING-SYSTEM FOR ON LINE ESTIMATION IN MAN OF BLOOD-GLUCOSE CONCENTRATION USING A MINIATURIZED GLUCOSE SENSOR IMPLANTED IN THE SUBCUTANEOUS TISSUE AND A WEARABLE CONTROL UNIT [J].
POITOUT, V ;
MOATTISIRAT, D ;
REACH, G ;
ZHANG, Y ;
WILSON, GS ;
LEMONNIER, F ;
KLEIN, JC .
DIABETOLOGIA, 1993, 36 (07) :658-663
[6]  
SASSO S, 1990, ANAL CHEM, V62, P2566
[7]   PROTEIN-MODIFIED ELECTRODES - THE GLUCOSE-OXIDASE POLYPYRROLE SYSTEM [J].
UMANA, M ;
WALLER, J .
ANALYTICAL CHEMISTRY, 1986, 58 (14) :2979-2983
[8]  
YASUZAWA M, 1993, SENSOR ACTUAT B-CHEM, V13, P665
[9]  
YASUZAWA M, 1997, P 3 E AS C CHEM SENS, P355
[10]  
YASUZAWA M, 1993, P EL SOC, V93, P808