Identification of peptides binding to IgG in the CSF of multiple sclerosis patients

被引:17
作者
Cortese, I
Capone, S
Tafi, R
Grimaldi, LME
Nicosia, A
Cortese, R [1 ]
机构
[1] Univ Rome, Neurol Clin, I-00100 Rome, Italy
[2] Osped Riabilitaz S Lucio, I-00173 Rome, Italy
[3] Ist Ric Biol Mol P Angeletti, I-00040 Pomezia, RM, Italy
[4] Univ Milan, Dept Neurol, DIBIT, Neuroimmunol Unit, I-20132 Milan, Italy
[5] Osped S Raffaele, I-20132 Milan, Italy
来源
MULTIPLE SCLEROSIS | 1998年 / 4卷 / 01期
关键词
multiple sclerosis; oligoclonal bonds; CSF; phage-libraries;
D O I
10.1191/135245898678909132
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Phage displayed random Peptide libraries were screened in order to identify phagotopes reacting with the IgG present in the cerebrospinal fluid (CSF) of multiple sclerosis (MS) patients. Six families of phagotopes each composed of different isolates were identified. These phagotopes were used as reagents, to characterise IgG present in the CSF of MS patients. The following results were obtained: (a) CSF antibodies from different Patients display different specificities; (b) anti-phagotope antibodies ore also Present in the serum of MS Patients, (c) Anti-phagotope antibodies ore equally frequent in the serum of MS patients and of healthy individuals; (d) some of the anti-phagotope antibodies ore enriched in the CSF of MS patients. These data show that the natural antigen(s) recognised by CSF antibodies is rather common in the general population. By using the selected phagotopes as immunogens in rabbits, we have derived large quantities of anti-phagotope antisera which con Provide for useful tools toward the identification of the natural antigen(s) recognised by MS CSF antibodies.
引用
收藏
页码:31 / 36
页数:6
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