Extraction and radioimmunoassay quantitation of neuropeptide Y (NPY) and vasoactive intestinal polypeptide (VIP) from human dental pulp tissue

被引：16

作者：

El-Karim, I ^{[1
]}

Lundy, FT ^{[1
]}

Linden, GJ ^{[1
]}

Lamey, PJ ^{[1
]}

机构：

[1] Queens Univ Belfast, Sch Dent, Oral Sci Res Ctr, Belfast BT12 6BP, Antrim, North Ireland

来源：

ARCHIVES OF ORAL BIOLOGY | 2003年 / 48卷 / 03期

关键词：

human; dental pulp; neuropeptide Y; radioimmunoassay; vasoactive intestinal polypeptide;

D O I：

10.1016/S0003-9969(02)00213-3

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

The measurement of neuropeptides in complex biological tissue samples requires efficient and appropriate extraction methods so that immunoreactivity is retained for subsequent radioimmunoassay detection. Since neuropeptides differ in their molecular mass, charge and hydrophobicity, no single method will suffice for the optimal extraction of various neuropeptides. In this study, dental pulp tissue was obtained from 30 human non-carious teeth. Of the three different neuropeptide extraction methods employed, boiling in acetic acid in the presence of protease inhibitors yielded the highest levels of neuropeptide Y (NPY) and vasoactive intestinal polypeptide (VIP). High pressure liquid chromatography (HPLC) analysis of dental pulp tissue verified the authenticity of the neuropeptides extracted. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：249 / 254

页数：6

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