Transforming activity of purinergic receptor P2Y, G-protein coupled, 2 revealed by retroviral expression screening

被引：13

作者：

Hatanaka, Hisashi

Takada, Shuji

Choi, Young Lim

Fujiwara, Shin-ichiro

Soda, Manabu

Enomoto, Munehiro

Kurashina, Kentaro

Watanabe, Hideki

Yamashita, Yoshihiro

Sugano, Kentaro

Mano, Hiroyuki

机构：

[1] Jichi Med Univ, Div Funct Genom, Shimotsuke, Tochigi 3290498, Japan

[2] Jichi Med Univ, Div Gastroenterol, Shimotsuke, Tochigi 3290498, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 356卷 / 03期

关键词：

purinergic receptor; oncogene; colorectal carcinoma; cDNA library; retrovirus;

D O I：

10.1016/j.bbrc.2007.03.048

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Colorectal cancer (CRC) is one of the leading causes of cancer death in humans. In order to identify novel cancer-promoting genes in CRC, we here constructed a retroviral cDNA expression library from a CRC cell line RKO, and used it for a focus formation assay with mouse 3T3 fibroblasts, leading to the identification of 42 independent cDNAs. One of such cDNAs turned out to encode purinergic receptor P2Y, G-protein coupled, 2 (P2RY2). The oncogenic potential of P2RY2 was confirmed in vitro with the focus formation assay as well as soft agar-growth assay, and also in vivo with a tumorigenicity assay in nude mice. While our P2RY2 cDNA encodes a protein with two amino-acid substitutions compared to the reported one, we have confirmed that the wild-type P2RY2 has a strong transforming potential as well. These results indicate an unexpected role of P2RY2 in the carcinogenesis of human cancers. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：723 / 726

页数：4

共 16 条

[11] Extracellular nucleotides inhibit growth of human oesophageal cancer cells via P2Y2-receptors [J].